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MultiScribe™ Reverse Transcriptase - FAQs

View additional product information for MultiScribe™ Reverse Transcriptase - FAQs (4311235)

3 product FAQs found

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

Can I purchase the MultiScribe Reverse Transcriptase from the TaqMan® MicroRNA Reverse Transcription Kit (Cat. No 4366596) separately?

Yes, the MultiScribe Reverse Transcriptase (Cat. No. 4311235) can be purchased as a standalone product. Please see the following link.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Does Applied Biosystems MultiScribe Reverse Transcriptase have RNase H activity?

Yes, MultiScribe Reverse Transcriptase (Cat. No. 4311235) does have RNase H activity.