AmpFLSTR™ Identifiler™ PCR Amplification Kit

Catalog number: 4322288

Applied Biosystems™  Related applications: PCR Amplification for Human Identification

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The AmpFLSTR™ Identifiler™ PCR Amplification Kit is the single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems™. The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards.

Features of the The AmpFLSTR™ Identifiler™ PCR Amplification Kit:

• Fifteen STR (short tandem repeat) loci and Amelogenin co-amplified in a single tube.
• Loci consistent with all major worldwide STR standards.
• Degenerate unlabeled primer addresses mutations observed in a population of Chamorros and Filipinos from Guam
• Includes 13 core loci required for sample entry in CODIS

Complete Validated Solution
Applied Biosystems offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR™ STR kits, instrumentation, software, and consumables. The Identifiler kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

Five Dye Technology Allows Enhanced Throughput
Identifiler utilizes the most advanced 5-dye technology to allow for the multiplex of more loci in a single lane or injection helping to increase your throughput and to simplify the workflow needed to obtain probative results. Our PET™ and LIZ™ -dyes were specifically designed to expand the spectral range of the 4-dye system. We have expanded the spectral range to 660 nm allowing for maximum color separation with minimal spectral overlap. As with all of our products, the five-dye technology is validated for use with our latest instruments and software as a complete system.

Experience Matters™
When you partner with us you gain access to the most extensive Human Identification resource network found within the industry. Our dedicated team of industry leading experts includes Research and Development scientists with extensive forensic product development experience, skilled Field Applications Support Scientists with over 100 years of combined forensic experience, and dedicated Field Service Engineers that provide on-site technical assistance with instrumentation.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For Forensic or Paternity Use Only.


Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, FAM™, PET™, LIZ™, VIC®
Product Size: 200 reactions
Amplicon Size: <360 bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
For Use With (Equipment): GeneAmp® 9700, 3500xL Genetic Analyzer, GeneAmp® 9600, 3500 Genetic Analyzer, 310 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130xl Genetic Analyzer, 3730 DNA Analyzer
Matrix and Size Standards: Matrix Standard Set DS-33, GeneScan™ 500 LIZ® Size Standard
Shipping Condition: Dry Ice

Contents & storage

• AmpFLSTR™ Identifiler PCR Reaction Mix
• AmpFLSTR™ Identifiler Primer Set
• AmpFLSTR™ Control DNA 9947A
Store at 2 to 8°C.

• AmpliTaq™ Gold DNA Polymerase
Store at -15 to -25 °C.

• AmpFLSTR™ Identifiler™ Allelic Ladder
Store at -15 to -25°C upon receipt, 2 to 8°C after initial use


Manuals & protocols

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