Applied Biosystems™

ABI PRISM™ 7000 Sequence Detection Systems Spectral Calibration Kit

Catalog number:  4328895

 Related applications:

Real Time PCR (qPCR)

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This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™ dyes on the ABI PRISM® 7000 Sequence Detection System. The ABI PRISM® 7000 Sequence Detection Systems Spectral Calibration Kit includes eight optical 96-well reaction plates: one preloaded and sealed background plate, and seven preloaded and sealed spectral calibration plates with seven separate dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™) are provided for quick and easy calibration of the ABI PRISM® 7000 Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the ABI PRISM® 7000 Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.
For Research Use Only. Not for use in diagnostic procedures.


Calibration Type: Spectral Calibration
Detection Method: Fluorescent
For Use With (Equipment): 7000 System
Label or Dye: FAM, JOE, NED, ROX, SYBR® Green, TAMRA™, TMR (Tetramethylrhodamine), VIC
Product Line: ABI PRISM™
Product Size: 8 x 96 well plates
Shipping Condition: Dry Ice

Contents & storage

Contains one Background plate, and seven Spectral Calibration plates with seven dye standards (FAM™, JOE™, NED™,ROX™, SYBR™ Green, VIC™, TAMRA™). Store at -15 to 25°C. Protect from light.


Manuals & protocols