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Applied Biosystems™

7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory

Catalog number: 4329002
Applied Biosystems™

7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory

Catalog number: 4329002
Catalog Number
4329002
Unit Size
1 instrument
Price (USD)
Catalog NumberUnit SizePrice (USD)
43290021 instrument
Product Overview
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The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. The optional, integrated Automation Accessory provides maximum throughput. Up to eighty-four 384-well plates can be loaded at a single time for unattended operation; a bar code reader allows you to insert or substitute plates into the sample queue at any time.

• Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate
• Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction
• Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences
• 96- or 384-well plate compatibility (including the TaqMan® Low Density Array)
• Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security
• Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab's productivity
• Proven assay development guidelines save time and money

High Throughput
The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An optional Automation Accessory combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of today's drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.

Flexibility
The 7900HT system is a versatile research tool that can accommodate any real-time PCR need. User-interchangeable thermal cycling block formats let you select the format that’s right for your project, using industry-standard 96- and 384-well formats, as well as a novel 384-well TaqMan® Low Density Array and a new Fast 96-well block that reduces run times from 2 hours to about 30 minutes. An easy automation upgrade path lets you add features to meet throughput demands. A Fast PCR option reduces run times from 2 hours to about 30 minutes.

Powerful Software
Using two automation software tools, Plate Utility and Automation Controller, most of the gene expression and SNP genotyping workflow is fully automated for high-throughput applications and requires minimal user intervention. You can now process 5,000 x 384 well plates (384 markers), which equates to 1.92 million genotypes. Using Relative Quantification (RQ) Manager, you can also process 200 x 384-well plates (96 detectors with quadruplicate data points⁄multiplexed endogenous control), which equates to 153,600 data points. Fully integrated into one complete Enterprise system, SNP Manager and RQ Manager eliminate data analysis bottlenecks in high-throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously. This reduces hands-on analysis time and simplifies the data analysis workflow.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)
7900HT Fast System, AB 7900 HT 384-Well System
Reaction Speed
Fast
Dynamic Range
Up to 9 logs of linear dynamic range
Sensitivity
Down to 10 copies
Capacity
Up to 84 x 384-well plates
Calibrated Dye
SYBR™ Green I, ROX™, NED™, JOE™, TAMRA™, VIC™, FAM™, TET™
Dimensions
72 cm (W) x 84 cm (D) x 64 cm (H) (with drawer open)
Detection Method
SYBR, Primer-Probe Detection
Format
384-well plate
High-throughput Compatibility
High-throughput Compatible, Multiplexing
Passive Reference Dye
ROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
Optics
Extended-life 488 nm argon-ion laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500 -650 nm)
Run Time
52 minutes (384-well in fast mode), <2 hrs (384-well in standard mode)
Reaction Volume Range
5-20 µl
Sample Ramp Rate
Fast mode: ± 3.0°C⁄sec, Standard mode: ± 1.6°C⁄sec
Temperature Range (Metric)
4-100°C
Temperature Uniformity
±0.5°C
Temperature Accuracy
±0.25°C of display temperature
Throughput
≥5,000 real-time PCR rxns per day (384-well block and automation accessory), ≥10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
Thermal Cycling System
Peltier-Based System
Weight
114 kg (250 lbs) with automation accessory
Block Format
Interchangeable
Includes
1- Instrument with 384-Well Block Module, Automation Accessory

Contents & Storage

1 Instrument with 384-Well Block Module and Automation Accessory

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Frequently asked questions (FAQs)

Citations & References