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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited), 500 Reactions - FAQs

Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited), 500 Reactions - FAQs

View additional product information for Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited) - FAQs (4333760T, 4352930E, 4333760F)

4 product FAQs found

I am working on both mouse and human samples for gene expression studies. Do you have one endogenous control that I can use with both species?

The 18S rRNA is highly conserved among eukaryotes. We have several configurations of assays for this gene: P/N 4331182, Assay ID: Hs99999901_s1; PN 4319413E, 4310893E, 4333760T, 4333760F, 4308329. You can use any of these for both human and mouse samples, as well as samples from any other eukaryotic species.

What are the key features of a TaqMan MGB probe?

TaqMan MGB probes have a nonfluorescent quencher at the 3´ end and a minor groove binder at the 3´ end that increases the melting temperature (Tm) of probe, allowing the use of shorter probes.  For more information on MGB probes, please consult the following manual: "Primer Express v1.5 and TaqMan MGB Probes for Allelic Discrimination: All PCR Instruments: User Bulletin." You can find a copy on our website by entering this title in the main search box.

When using the TaqMan Gene Signature Plates, should I mix the reactions after adding the master mix and template to the wells containing lyophilized primers and probe?

No, but it is recommended that the plate be centrifuged to draw down the liquid to the bottom of the wells.

What are your recommendations for the best way to dilute and store my TaqMan probe?

The probe should be diluted in TE buffer [10 mM Tris-HCl, pH 8.0 (at 25°C), 1 mM EDTA]. We recommend storing your probes, both stocks and working solutions, at -20°C in aliquots. This is to cut down on the number of times the probe is subject to freeze-thaws. For detailed information on diluting primers and probes, please refer to the tutorial entitled "Reconstituting and Diluting Primers and TaqMan Probes". You can find it on our website by entering this title in the main search field.

Find additional tips, troubleshooting help, and resources within ourTaqMan Primers and Probes Support Center.