POP-7™ Polymer for 3730/3730xl DNA Analyzers

You can run 8,000-12,000 samples. These numbers may be lower if there is increased polymer consumption due to additional wizards being performed, such as the Bubble Remove and Water Wash wizards.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

POP-7 polymer can maintain its performance for seven days on the Applied Biosystems 3730 Series DNA Analyzer at standard lab conditions. After seven days, perform all required maintenance (including the Water Wash Wizard) and replace with fresh polymer. Do not "top off" the polymer bottle (add fresh polymer to old polymer), which can adversely affect data quality and consumable lifespan.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

Applied Biosystems supports the use of POP-7 polymer on the Applied Biosystems 3730 Series DNA Analyzer. If the instrument is running Applied Biosystems 3730 Data Collection Software v.3.1 or later, then you can also use POP-6 polymer. We do not recommend mixing and matching of various instrument polymers as each is manufactured in different processes, tested as an entire system, and guaranteed to perform to a minimum specification. Substitution of reagents may compromise the integrity of the system and cannot be supported by AB service or applications support specialists. Using unsupported polymers may adversely affect data quality, consumable lifespan, and may void existing warranties.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

When placing the array on the instrument, the last step in the process is to loosen the array knob and the instrument forces any air bubbles around the array tip through the threading and the middle of the array knob. Some polymer gets pushed through as well and when it dries up, it appears as a white, flaky residue. Use a damp, lint-free cloth to remove it. If more residue appears after the cleanup, it is possible that the array knob is not tightened enough or the array ferrule may require replacement.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

It is not unusual to see a large bubble at the tip of the piston while performing the water wash wizard on the system. When the polymer is put back on to the system, the difference in viscosity is usually enough to dislodge the bubble and push it out. In extreme cases, it may be necessary to repeat the wash and go back and forth between the water and polymer to force it out.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.