BigDye® Terminator v1.1 Cycle Sequencing Kit

Catalog number: 4337449

Applied Biosystems™  Related applications: Sanger Sequencing

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This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications.
• Sequence challenging templates and sequences more successfully.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Tackle Tough Sequences
With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow
BigDye® Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): GeneAmp™ 9700, 3700 Genetic Analyzer, GeneAmp® 9600, GeneAmp® 2700, 3130xl Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3100 Genetic Analyzer, 310 Genetic Analyzer, ABI PRISM™ 377, GeneAmp™ 2400
Recommended Applications: AT-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Short PCR Product Sequencing, GC-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), De Novo Sequencing - Low to Mid Throughput
Product Size: 24 reactions

Contents & storage

200µL Tube of BigDye® Terminator v1.1 Ready Reaction Mix, Tube M13 (-21) Primer, Tube pGEM Control DNA, 1mL Tube of 5x Sequencing Buffer. Store kit at -15 to -25°C. The BigDye® sequencing buffer can be stored at 4°C.


Manuals & protocols