Applied Biosystems™

BigDye™ Terminator v1.1 Cycle Sequencing Kit

Catalog number:  4337449

Related applications:

Sanger Sequencing

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This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications.
• Sequence challenging templates and sequences more successfully.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Tackle Tough Sequences
With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow
BigDye® Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Product Line: BigDye® Terminator
Recommended Applications: AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), Short PCR Product Sequencing, GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, De Novo Sequencing - Low to Mid Throughput
For Use With (Equipment): 310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3700 Genetic Analyzer, ABI PRISM™ 377, GeneAmp 2700, GeneAmp 9600, GeneAmp 9700, GeneAmp™ 2400
Description: Applied Biosystems™ BigDye™ Terminator v1.1 Cycle Sequencing Kit, 20 rxns, Optimal Base calling Adjacent to the Primer and For Sequencing Short PCR Product Templates with Rapid Electrophoresis Run Modules

Contents & storage

200µL Tube of BigDye® Terminator v1.1 Ready Reaction Mix, Tube M13 (-21) Primer, Tube pGEM Control DNA, 1mL Tube of 5x Sequencing Buffer. Store kit at -15 to -25°C. The BigDye® sequencing buffer can be stored at 4°C.