BigDye® Terminator v1.1 Cycle Sequencing Kit

Catalog number: 4337451

Applied Biosystems™  Related applications: DNA Sequencing | Sanger Sequencing

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Description

This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications
• Sequence challenging templates and sequences more successfully
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Tackle Tough Sequences
With the BigDye® Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye® Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): GeneAmp® 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, Veriti® Thermal Cycler, 3500 Genetic Analyzer, 3130xl Genetic Analyzer, 310 Genetic Analyzer, 3130 Genetic Analyzer, 3730xl DNA Analyzer
Recommended Applications: Standard Sequencing
Format: 8-well strips, 12-strip wells, 96-well plate, 384-well plate
Technique: Fluorescent Dye Terminator Sequencing
Product Size: 1,000 reactions
Template Compatibility: BAC DNA, Single Stranded DNA, Plasmid DNA (≤15Kb), PCR Amplicons

Contents & storage

Each kit contains 10 tubes of BigDye® Terminator v1.1 Ready Reaction Mix, M13 (-21) Primer, pGEM Control DNA, and a 12-mL tube of 5X Sequencing Buffer (may be stored at 4°C). There are enough reagents for 1000 reactions. Store kit at -15°C to -25°C. Avoid repeated freeze-thaw cycles.

Documents

Manuals & protocols