Applied Biosystems™

BigDye™ Terminator v3.1 Cycle Sequencing Kit

Catalog number:  4337454

 Related applications: Sanger Sequencing

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The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications.
• Optimized for long read lengths.
• Better dye mobility characteristics
• Improved performance reading through GT rich regions.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): 310 Genetic Analyzer, 3130 Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti Thermal Cycler
Recommended Applications: AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), De Novo Sequencing - High Throughput (cDNA), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Long-Read Sequencing, Mixed-Base Detection, Resequencing
Product Line: BigDye® Terminator
Product Size: 24 reactions
Template Compatibility: BAC DNA, Fosmids, Genomic DNA (Bacterial), Lambda DNA, PCR Amplicons, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), Rolling Circle Amplified Products, Single Stranded DNA

Contents & storage

200µl tube of BigDye® Terminator v3.1 Ready Reaction Mix, 1 tube M13 (-21) Primer, 1 tube pGEM Control DNA, and a 1 ml tube of 5X Sequencing Buffer. Store kit at -15°C to -25°C. The BigDye® sequencing buffer can be stored at 4°C.


Manuals & protocols