Applied Biosystems™

BigDye™ Terminator v3.1 Cycle Sequencing Kit

Catalog number:  4337454

 Related applications: Sanger Sequencing

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Description

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications.
• Optimized for long read lengths.
• Better dye mobility characteristics
• Improved performance reading through GT rich regions.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): 310 Genetic Analyzer, 3130 Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler
Recommended Applications: AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), De Novo Sequencing - High Throughput (cDNA), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Long-Read Sequencing, Mixed-Base Detection, Resequencing
Product Line: BigDye® Terminator
Product Size: 24 reactions
Template Compatibility: BAC DNA, Fosmids, Genomic DNA (Bacterial), Lambda DNA, PCR Amplicons, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), Rolling Circle Amplified Products, Single Stranded DNA

Contents & storage

200µl tube of BigDye® Terminator v3.1 Ready Reaction Mix, 1 tube M13 (-21) Primer, 1 tube pGEM Control DNA, and a 1 ml tube of 5X Sequencing Buffer. Store kit at -15°C to -25°C. The BigDye® sequencing buffer can be stored at 4°C.

Documents

Manuals & protocols