The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT-rich regions • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)
Sequencing
Product Type
Sequencing Buffer
Quantity
24 Reactions
Description
Array Install Kit, 96 x 36cm Cap, For 3730XL Genetic Analyzer
Format
Kit
Includes
200μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix, 1 tube M13 (-21) Primer, 1 tube pGEM Control DNA, and a 1mL tube of 5X Sequencing Buffer
Recommended Applications
AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
Template Compatibility
BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), PCR Amplicons
200µl tube of BigDye™ Terminator v3.1 Ready Reaction Mix, 1 tube M13 (-21) Primer, 1 tube pGEM Control DNA, and a 1 ml tube of 5X Sequencing Buffer. Store kit at -15°C to -25°C. The BigDye™ sequencing buffer can be stored at 4°C.
