Applied Biosystems™
BigDye™ Terminator v3.1 Cycle Sequencing Kit
Description
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| For Use With (Equipment): | 310 Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer |
|---|---|
| Description: | Array Install Kit, 96 x 36cm Cap, For 3730XL Genetic Analyzer |
| Includes: | 200μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix, 1 tube M13 (-21) Primer, 1 tube pGEM Control DNA, and a 1mL tube of 5X Sequencing Buffer |
| Recommended Applications: | AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing |
| Template Compatibility: | BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15Kb), PCR Amplicons |
| Product Line: | BigDye® Terminator |
Contents & storage
200µl tube of BigDye® Terminator v3.1 Ready Reaction Mix, 1 tube M13 (-21) Primer, 1 tube pGEM Control DNA, and a 1 ml tube of 5X Sequencing Buffer. Store kit at -15°C to -25°C. The BigDye® sequencing buffer can be stored at 4°C.
