BigDye™ Terminator v3.1 Cycle Sequencing Kit
Notice: We're enhancing our manufacturing process for DNA Control Template, now labelled as pREF-BDT Control DNA in kit, for consistent high-quality delivery. No changes to preparation or storage conditions will occur ensuring seamless integration into your current workflows. Please see the product user guide for more information.
BigDye™ Terminator v3.1 Cycle Sequencing Kit
Applied Biosystems™

BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishingRead more
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Catalog NumberQuantity
4337455100 Reactions
433745424 Reactions
43374561000 Reactions
43374575000 Reactions
433745825,000 Reactions
Catalog number 4337455
Price (USD)/ 100 reactions
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Quantity:
100 Reactions

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

  • Improve the quality of your results for a wide range of sequencing applications
  • Optimized for long read lengths
  • Better dye mobility characteristics
  • Improved performance reading through GT-rich regions
  • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
  • Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy

With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format12-well Strips, 8-well Strip, 96- or 384-well Plate
For Use With (Equipment)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
For Use With (Application)AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
Product LineBigDye™ Terminator
Product TypeSequencing Buffer
Quantity100 Reactions
TechniqueFluorescent Dye Terminator Sequencing
Template CompatibilityBAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons
Unit Size100 reactions
Contents & Storage
• 1 x 800 μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix
• 1 tube M13 (-21) Primer
• 1 tube pREF-BDT™ Control DNA
• 2 x 1 mL tubes of 5X Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.