Applied Biosystems™

7500 Real Time PCR Systems Spectral Calibration Kit I This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  4349180

 Related applications:

Real Time PCR (qPCR)

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dyes on the 7500 Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit I contains one background plate, one region of interest (ROI) calibration plate, and seven spectral calibration plates with seven separate dye standards.

• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/ROX™) are provided for quick and easy calibration of the 7500 Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 7500 Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.
For Research Use Only. Not for use in diagnostic procedures.


Calibration Type: Spectral Calibration
Detection Method: Fluorescent
For Use With (Equipment): 7500 System
Label or Dye: FAM, JOE, NED, ROX, SYBR® Green, TAMRA™, VIC
Product Size: 1 kit
Green Features: Sustainable packaging
Shipping Condition: Dry Ice

Contents & storage

Includes one background plate, seven spectral calibration plates with FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dye standards, and one ROI calibration plate.

Store at -15°C to -25°C. Protect from light.


Manuals & protocols