TaqMan® E. coli O157:H7 Detection Kit - FAQs

The TaqMan Escherichia coli O157:H7 Detection Kit has demonstrated 100% inclusivity for 16 strains of serotypes 0157:H7 and 055:H7. It has also demonstrated 100% exclusivity against 31 other Escherichia coli strains and 21 non-Escherichia coli strains. For more specific information, please refer to “Appendix A: Specificity” of the TaqMan E. coli 0157:H7 Detection Kit Protocol. Also, included there is the American Tissue Culture and Collection Strains / Source information.

Each individual component in the TaqMan Pathogen Detection Kits is color-coded in order to best distinguish between the 2X Environmental Master Mix, the negative control and the 10X Target Assay Mix (TAM). Yellow coded vials are 2X Environmental Master Mix, the white coded vial is the negative control solution, and the 10X Target Assay Mix (TAM) which is associated with a specific organism is a gray-labeled vial.