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TaqMan® Listeria monocytogenes Detection Kit

Catalog number: 4366102

Applied Biosystems™  Related applications: Diagnostic Testing | Food & Beverage

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The TaqMan® Listeria monocytogenes Detection Kit provides a simple, reliable, and rapid procedure that utilizes the polymerase chain reaction (PCR) to amplify a target sequence unique to Listeria monocytogenes.

• Reduce sample handling with a software-guided, 3-step process that uses a single, closed-tube format during the PCR step.
• A highly sensitive TaqMan® Real-Time PCR system dramatically reduces the time required for pre-enrichment compared to conventional techniques.
• Get your results quickly with time to results of less than 4 hours after sample enrichment (28 hours total).
• Detect 1-10 copies of the target DNA per reaction-or down to 1 colony-forming unit in 25 grams of food.
• Streamline your workflow with one standard protocol for multiple organisms.

3 Easy Steps
This kit has 3 easy steps: enrich samples, prepare samples, and run samples. Grow the specific pathogen from a selected sample using a standard enrichment procedure in the enrichment step. In the sample preparation step isolate the DNA for PCR, and prepare, mix and pipet the reactions. In the final step, analyze your sample by running a plate on one of our real-time PCR systems.

Clear Results
RapidFinder™ software guides you through the assay procedure, and a comprehensive online help feature provides immediate support whenever required. Minutes after detection is complete, results are displayed in an easy-to-read display for fast, accurate interpretation. Results can be printed out in a flexible report format if required. You can also view basic troubleshooting suggestions.

High Specificity
The kit demonstrates 100% inclusivity for 29 strains of Listeria monocytogenes and 100% exclusivity for 37 other non-Listeria and non-monocytogenes strains.

A Complete Solution
We offer a full line of TaqMan® Pathogen Detection kits based on universal assay conditions, enabling you to run any combination of these assays together in the same sample run. In combination with our real-time PCR systems, the TaqMan® Listeria monocytogenes Detection Kit offers a fully integrated, single-vendor solution to identify Listeria monocytogenes.

For Research Use Only. Not for use in diagnostics procedures.

Contents & storage

This kit contains Target Assay Mix (store at -20°C in the dark), a Negative Control (store at -2 to 8°C), and 2X Environmental Master Mix (store at -2 to 8°C).

There are enough reagents for 100 assays. These reagents are stable for one year after shipment.


Manuals & protocols