High-Capacity cDNA Reverse Transcription Kit, 1000 reactions - FAQs

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

• High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
• High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).

Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?

Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What is the concentration of the random primers in the High-Capacity cDNA Reverse Transcription Kit (Cat. no. 4368813)?

The concentration of the "random primers" in the High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813) is 25 µM. We recommend that you use these primers at a 1X concentration (2.5 µM) in reverse transcription reactions.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

Is the 25X 100 mM dNTP Mix included in the High Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813, 4368814) 100 mM each or 100 mM combined?

It contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).