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TaqMan® Yersinia pestis Detection Kit

Catalog number: 4382488

Applied Biosystems™  Related applications: Diagnostic Testing

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Description

Applied Biosystems® TaqMan® Yersinia pestis Detection Kit uses the polymerase chain reaction (PCR) to amplify a unique microorganism specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. The TaqMan® Yersinia pestis Detection Kit provides a simple, reliable, and rapid procedure that utilizes the polymerase chain reaction (PCR) to amplify a target sequence unique to Yersinia pestis
• Reduce sample handling with a 3-step process that uses a single, closed-tube format during the PCR step.
• Highly sensitive TaqMan® Real-Time PCR system enables a dramatic reduction in time required for pre-enrichment compared to conventional techniques.
• Streamline your workflow with one standard protocol for multiple organisms.

3 Easy Steps
This kit has 3 easy steps: enrich samples, prepare samples, and run samples. Grow the specific pathogen from a selected sample using a standard enrichment procedure in the enrichment step. In the sample preparation step isolate the DNA for PCR, and prepare, mix and pipet the reactions. In the final step, analyze your sample by running a plate on one of our real-time PCR systems.

A Complete Solution
We offer a full line of TaqMan® Pathogen Detection kits based on universal assay conditions, enabling you to run any combination of these assays together in the same sample run. In combination with our real-time PCR systems, the TaqMan® Yersinia pestis Detection Kit offers a fully integrated, single-vendor solution to identify Yersinia pestis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Organism Class: Y. pestis [Plague]
Validated Application: Real-Time PCR
Detection Method: Primer-Probe Detection
Label or Dye: FAM™
Product Size: 100 reactions
Target Molecule: Bacteria
Shipping Condition: Dry Ice
Target Specificity: Target-Specific

Contents & storage

300µL 10X Yersinia pestis Target Assay CAF1 (store at -20°C), 300µL 10X Yersinia pestis Target Assay PLA (store at -20°C), 1,000µL Negative Control (store at 2°C-8°C), Two Tubes 2X Fast PCR Master Mix 1,500µL (store at 2°C-8°C).

Documents

Manuals & protocols