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      TaqMan™ Cronobacter sakazakii Detection Kit

      Catalog number:  4382492

      Applied Biosystems™  Related applications: Diagnostic Testing | Food & Beverage

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      Description

      The TaqMan® Cronobacter sakazakii Detection Kit provides a simple, reliable, and rapid procedure for detecting the presence of Cronobacter sakazakii. The assay utilizes the polymerase chain reaction (PCR) to amplify a target unique to that microorganism and TaqMan® probes that identify the specific organism.

      • Highly selective identification of C. sakazakii in a wide variety of samples.
      • Verified performance
      • Ready-to-use convenience
      • Minimal risk of contamination

      3 Easy Steps
      This kit has 3 easy steps: Enrich samples, prepare samples, and run samples. Grow the specific pathogen from a selected sample using a standard enrichment procedure in the Enrichment step. In the Sample Preparation step isolate the DNA for PCR, and prepare, mix and pipet the reactions. The final step is Running a Plate on an Applied Biosystems® SDS or Real-Time PCR System to analyze your sample.

      A Complete Solution
      We offer a full line of TaqMan® Pathogen Detection kits based on universal assay conditions, enabling you to run any combination of these assays together in the same sample run. In combination with our real-time PCR systems, the TaqMan® Cronobacter sakazakii Detection Kit offers a fully integrated, single-vendor solution to identify Cronobacter sakazakii.

      For Research Use Only. Not for use in diagnostics procedures.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Target Organism Class: C. sakazakii
      Validated Application: Real-Time PCR
      Detection Method: Primer-Probe Detection
      PCR Method: qPCR
      For Use With (Equipment): 7300 System, 7500 System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
      Sample Type (General): Bacteria, Food & Environmental Samples
      Sample Type (Specific): Any Swab Type, Food, Water Samples
      Hot Start: Built-In Hot Start
      Passive Reference Dye: ROX (Pre-mixed)
      Reaction Speed: Standard
      Primer-Probe Compatibility: TaqMan®
      Polymerase: AmpliTaq Gold® DNA Polymerase
      Number of Reactions: 100 Tests
      Product Line: TaqMan™
      Product Size: 1 kit
      Shipping Condition: Dry Ice

      Contents & storage

      This kit contains 10X Cronobacter sakazakii target assay mix (store at -20°C in the dark), a Negative Control (store at -2 to 8°C), and 2X Environmental Master Mix (store at -2 to 8°C).

      There are enough reagents to perform 100 assays. These reagents are stable for one year after shipment.

      Documents

      Manuals & protocols