Veriti™ 60-well Thermal Cycler (for 0.5 mL tubes) - FAQs

If the test fails:

1.Verify that the:
-Calibration of the TVK is up-to-date.
-Probe tips are not cracked or damaged.
2.Dispense 2 to 3 drops of mineral oil onto a cotton swab. Use the swab to apply a thin film of oil to the cone surface of each probe to improve heat transfer.
3.Repeat the steps in “Before You Run 96-Well Instrument Tests” from the Multiprobe Temperature Verification Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf).
4.Verify that the:
-Probes are seated securely in the appropriate test wells.
-Cables are connected securely to the ports.
-Ribbon cable lies flat under the thermal cutout and is not crimped.
-Heated cover is closed and locked down.
5.Repeat the steps in “Run the Test” from the Multiprobe Temperature Verification Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf). Please note, if the test fails again, contact us at instrumentservices@thermofisher.com.

This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).

Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)

Please refer to the guidelines in this protocol (https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) specific to the Veriti Thermal Cycler.

To add an infinite hold, select the infinity symbol for program.

The Veriti Thermal Cycler with a 96-well block (Fast and Standard) features innovative VeriFlex Blocks for enhanced PCR functionality. Six separate Peltier blocks provide maximal versatility and flexibility and offer two key benefits:
1. PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2. Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.

The 96-Well Block has a 3.9 degrees C/sec max block ramp rate, the 96-Well Fast Block has a 5.0 degress C/sec max block ramp rate, the 384- Well Block has a 3.7 degrees C/sec max block ramp rate, and the 60-Well Block has a 3.3 degrees C/sec max block ramp rate.

The plates and plastics will be specific to the block type. Choose the appropriate block type below for a list of the compatible tubes, plates, and films.

-Veriti 60-Well
-Veriti 96-Well 0.1 mL
-Veriti 96-Well 0.2 mL
-Veriti 384-Well

The Veriti Thermal Cycler is available in four formats:
Veriti 96-Well Thermal Cycler (a 0.2 mL or 0.1 mL)
Veriti 384-Well Thermal Cycler
Veriti 60-Well Thermal Cycler Veriti 96-Well Fast Thermal Cycler

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.