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Invitrogen™

SYBR™ Green Cells-to-CT™ Control Kit

Catalog number: 4402959
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Invitrogen™

SYBR™ Green Cells-to-CT™ Control Kit

Catalog number: 4402959
Have Questions?
Catalog Number
4402959
Unit Size
100 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4402959100 reactions
-
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This kit is designed for use with the Power SYBR™ Green (SKU#s 4402953, 4402954, and 4402955) and Fast SYBR™ Green Cells-to-CT™ Kits (SKU#s #4402956 and 4402957). Sufficient reagents are provided for 100 reactions. It includes Xeno™ RNA Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, and a PCR primer set for the Xeno™ RNA Control target. It also includes a PCR primer set for the highly expressed endogenous control gene β-actin (ACTB). Together these reagents provide positive controls for reverse transcription and SYBR™ Green-based real-time PCR, and can indicate the presence of any reverse transcription or PCR inhibitors. Real-time RT-PCR with SYBR™ ACTB Primers can also provide an endogenous control for sample normalization, as well as to confirm sufficient cell input in experiments with samples consisting of more than 100 cells per lysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Control Template
XenoRNA™ control (synthetic RNA transcript)
Format
Tube
Gene Symbol
ACTB
Green Features
Fewer resources used and less waste, Less hazardous
Label or Dye
Unconjugated
Product Line
Cells-to-CT™, SYBR™
Shipping Condition
Dry Ice
Target
XenoRNA™ control, β-actin
No. of Reactions
100 Reactions

Contents & Storage

Components:
• 100 μL Xeno™ RNA Control, 105 copies/μL
• 250 μL 20X SYBR Xeno Primers
• 250 μL 20X SYBR ACTB Primers

Amounts:
• Xeno RNA Control: approximately 100 lysis reactions
• SYBR Xeno and SYBR ACTB Primers: up to 250 x 20 μL PCRs

Storage Conditions: Store at −20°C. Do not store in a frost-free freezer.

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