SYBR® Green Cells-to-CT™ Control Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 4402959

Ambion™  Related applications: Real Time PCR (qPCR)

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This kit is designed for use with the Power SYBR® Green (SKU#s 4402953, 4402954, and 4402955) and Fast SYBR® Green Cells-to-CT™ Kits (SKU#s #4402956 and 4402957). Sufficient reagents are provided for 100 reactions. It includes Xeno™ RNA Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, and a PCR primer set for the Xeno™ RNA Control target. It also includes a PCR primer set for the highly expressed endogenous control gene β-actin (ACTB). Together these reagents provide positive controls for reverse transcription and SYBR® Green-based real-time PCR, and can indicate the presence of any reverse transcription or PCR inhibitors. Real-time RT-PCR with SYBR® ACTB Primers can also provide an endogenous control for sample normalization, as well as to confirm sufficient cell input in experiments with samples consisting of more than 100 cells per lysis.
For Research Use Only. Not for use in diagnostic procedures.


Format: Tube(s)
Includes: Control Primers & Template(s)
Gene Symbol: ACTB
Target Gene: β-actin, XenoRNA™ control
Label or Dye: Unconjugated
Product Size: 100 reactions
Control Template: XenoRNA™ control (synthetic RNA transcript)
3' Primer Modification: None
5' Primer Modification: None
Green Features: Less hazardous, Less waste, Fewer resources used
Shipping Condition: Dry Ice

Contents & storage


100 µL Xeno™ RNA Control, 10^5 copies/µL

250 µL 20X SYBR Xeno Primers

250 µL 20X SYBR ACTB Primers


Xeno RNA Control: approximately 100 lysis reactions

SYBR Xeno and SYBR ACTB Primers: up to 250 PCRs (20 µL) each

Storage Conditions: Store at −20°C. Do not store in a frost-free freezer.


Manuals & protocols

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