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MicroSEQ® Listeria monocytogenes Detection Kit

Catalog number: 4403874

Applied Biosystems™  Related applications: Diagnostic Testing | Food & Beverage

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This kit is recommended for users who have already purchased the starter kit but would like to perform more tests. The MicroSEQ® Listeria monocytogenes Detection Kit detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.
• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total)
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps:
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® Listeria monocytogenes Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 cfu per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder® Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria monocytogenes Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.
For Testing of Food and Environmental Samples Only.


Target Organism Class: L. monocytogenes
Validated Application: Real-Time PCR
Detection Method: Primer-Probe Detection
PCR Method: qPCR
For Use With (Equipment): 7500 Fast System, StepOnePlus™, Fast Mode, StepOne™, Fast Mode, StepOnePlus™
Sample Type (General): Bacteria, Food & Environmental Samples
Sample Type (Specific): Food, Water Samples, Any Swab Type
Hot Start: Built-In Hot Start
Reaction Speed: Fast
Passive Reference Dye: ROX (Pre-mixed)
Primer-Probe Compatibility: TaqMan®
Polymerase: AmpliTaq® Fast DNA Polymerase
Product Size: 1 kit
Number of Reactions: 96 Tests

Contents & storage

This kit includes 12 8-tube strips containing assay beads (store at 2 to 8°C in the dark), optical 8-tube strips and 8-cap strips (store at 22 to 28°C), and a Negative Control (store at 2 to 8°C).

These reagents are sufficient for 96 reactions.


Manuals & protocols