Silencer™ Select Pre-Designed siRNA - FAQs

View additional product information for Silencer™ Select Pre-Designed siRNA - FAQs (4404014)

2 product FAQs found

Where should I add a fluorescent tag to minimize any negative functional effect on my siRNA?

For any RNAi duplex (miRNA or siRNA) there is a possibility that adding a modification will affect how the antisense strand interacts with RISC and aligns with its mRNA target, thus impacting functionality. For that reason, we recommend that you label the 5' end of the sense strand for siRNA (or passenger strand for miRNA). Since the 5' end of the antisense (guide strand) is what gets recognized by RISC, it is best to add the fluorescent modification to the end of the duplex on the strand that does not interact with RISC. You can also test a labeled vs. non-labeled RNAi duplex to determine if the modification is affecting the siRNA or miRNA functionality.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How can I find the GC content for the predesigned Silencer siRNA or Silencer Select siRNA sequences prior to purchase?

Please contact Technical Support at techsupport@thermofisher.com to request the GC content for one of our predesigned Silencer siRNA or Silencer Select siRNA sequences.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.