TaqMan® Protein Expression Assay Kit (hCSTB)

Catalog number: 4405465

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Stem Cell Analysis

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Description

Applied Biosystems® TaqMan® Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan® Protein Expression Assay Kit (Human CSTB) contains the assay probes and a dilution buffer for the labelling of human cystatin-B protein (hCSTB) in cell lysates prepared using the Protein Expression Sample Preparation Kit. The assay probes consist of two oligonucleotides conjugated to antibodies that bind to separate epitopes of the hCSTB protein. Key features of the TaqMan® Protein Expression Assay:
• Quantitates protein expression using gold standard TaqMan® 5’ nuclease chemistry
• Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
• Allows greater sensitivity and a more quantitative readout than western blots and immunostaining
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Hot Start: No
Technique: Proximity Ligation Assay
PCR Method: qPCR Protein Quantification
Polymerase: AmpliTaq® Fast DNA Polymerase
Label or Dye: FAM™
Product Size: 100 reactions
Reaction Speed: Fast
Detection Method: Primer-Probe Detection
Target Specificity: Target-Specific
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (General): Cells
Purity or Quality Grade: UP (Ultra Pure)
For Use With (Equipment): 7500 Fast System, StepOnePlus™, Fast Mode, StepOne™, Fast Mode, 7900HT Fast System
Primer-Probe Compatibility: TaqMan®
Shipping Condition: Wet Ice

Contents & storage

Contains Assay Probe A, Assay Probe B, and Assay Probe Dilution Buffer. The assay probes (Assay Probe A and Assay Probe B) are supplied at a 20x concentration. The Assay Probe Dilution Buffer is supplied at a 1x concentration. Store at -15 to -25°C.

Documents

Manuals & protocols