Applied Biosystems™

3500xL Genetic Analyzer for Human Identification This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  4406016

 Related applications:


| Sanger Sequencing

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The 24-capillary 3500xL Genetic Analyzer sets the standard in capillary electrophoresis. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application. The complete product combines the instrument with Applied Biosystems™ reagents, consumables and software, as well as industry leading support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.

• Increased Consistency – Reduced signal variation with factory standardization and optional in-lane normalization
• Real-Time Data Quality Assessment – On instrument data evaluation features provide rapid identification and re-injection of samples requiring additional processing
• Improved Thermal Control – Smaller oven design and improved door seal for more consistent data migration
• HID Specific Workflow – Pre-configured for Applied Biosystems® amplification kits to simplify run setup and software navigation
• Effortless Consumable Installation – Convenient one-time use consumable packaging
• Consumable Tracking with RFID Technology – On-instrument, real-time monitoring of consumable consumption and expiration
• Enhanced Throughput – Faster data generation with 8 or 24 capillary arrays and shorter run times
• Improved Polymer Pump Design – Reduced waste and fewer bubbles for better data quality
• Simplified Instrument Installation & Operation – New solid-state laser eliminates need for high voltage wiring and external venting
• Streamlined Instrument Maintenance – HID specific performance check and maintenance scheduling tools
• Data Chain of Custody – Controlled access and tracking through Security, Audit and eSig features

For more information, please contact your Sales Representative.
For Forensic or Paternity Use Only.


Analysis Software: GeneMapper® ID-X Software
Capillary Length: 36 cm, 50 cm
Compatible Polymers: POP-4™, POP-6™, POP-7™
Current Limit: 15 amps
Dimensions: Electrophoresis Unit Width (Closed Door): 61 cm, Electrophoresis Unit: 122 cm (W) (open door) x 61 cm (D) x 72 cm (H)
External Computer: Hard Drive: 2x 500GB SATA 3.0 Gb/s and 8 MB Data Burst Cache, Memory: 16 GB (2x 8GB) 1600 MHz DDR3 Non-ECC. 4th Gen Intel Core I7 Processor- 3.1 GHz Turbo Processor, Operating System: Windows 7 SP1
For Use With (Equipment): 3500xL Genetic Analyzer
Format: 384-well plate, 8-well strips, 96-well plate
Green Features: Energy efficient, Fewer resources used and less waste
Laser Features: Long-life single-line 505 nm solid-state laser
Number of Capillaries: 24 Capillaries
Operating Environment: Humidity: 20%-80% (non-condensing), Room temp: <2° fluctuation during runs, Temperature: 15°C-30°C
Operating System: Windows 7 (Service Pack 1)
Plan Duration: 1 Year Limited Warranty on Parts and Labor, Service Installation and Application Training Included
Polymer Consumption: Replace at earliest of 14 days⁄384 samples⁄20 injections (384-sample pouches), Replace at earliest of 14 days⁄96 samples⁄5 injections (96-sample pouches), Replace at earliest of 14 days⁄960 samples⁄50 injections (960-sample pouches)
Product Size: 1 system
Read Length: ≥420 bp using a 36 cm capillary and POP-4™
Run Time: 30 to 140 min. (varies depending on run module)
Throughput: ≥ 936 samples/day (HID36_POP4), ≥264 samples per day (standard sequencing with POP-7™ and 50 cm capillaries), Range: 240 to 1104 sequencing samples per day (varies depending on run module)
Voltage Limit: Electrophoresis Voltage - Up to 20 kV
Weight: 82 kg (approximately)

Contents & storage

The 3500xL analyzer is shipped with the following system components:

• Capillary Electrophoresis instrument.
• 3500xL (24-capillary) array and POP™ polymer
• Human identification reagents and other consumables for system qualification.
• Dell® computer workstation with flat-screen monitor.
• Integrated software for instrument control, data collection, quality control, and basecalling or sizing of samples.


Manuals & protocols