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MicroSEQ® Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit

Catalog number: 4412637

Applied Biosystems™  Related applications: Food & Beverage

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Description

The MicroSEQ® Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is recommended for those needing to prepare and analyze hundreds of food or environmental samples per day. The Starter Kit detects Listeria monocytogenes simply, reliably, and rapidly using the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with the PrepSEQ® Nucleic Acid Extraction Kit. For ease of ordering, we have combined a 96 reaction MicroSEQ® Listeria monocytogenes Detection Kit with a 100 reaction PrepSEQ™ Nucleic Acid Extraction Kit and offer it here as a single product.

Features of the MicroSEQ® Listeria monocytogenes Detection Starter Kit:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software-guided 3-step process
• Time-to-results of less than 3 hours after sample enrichment (less than 27 hours total)
• Eliminate false negatives with internal positive control
• Detect 1–3 colony-forming units (CFU) per 25 grams of food
• Universal PCR cycling conditions for all MicroSEQ® pathogen detection kits
• Validated by the AOAC Research Institute

Optimized Sample Preparation
The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ® Nucleic Acid Extraction Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples for detection of 1–3 cfu per 25 grams of sample. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Universal Fast PCR Cycling Conditions
All MicroSEQ® pathogen detection kits utilize universal PCR cycling conditions which reduce error and allow maximum user flexibility. Fast PCR chemistries are completed in 40 minutes—saving time and clearing presumptive negatives quickly.

Clear Results using a Software-guided Procedure
After sample enrichment and preparation, RapidFinder® Express Software (available separately) guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow, including the MicroSEQ® Listeria monocytogenes Detection Kit, PrepSEQ® Nucleic Acid Extraction Kit, 7500 Fast instrument and RapidFinder™ Express software, has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ® Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is part of a complete food testing solution. The kit includes the reagents required to prepare up to 100 sample preparations and run 96 Listeria monocytogenes detection assays. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria monocytogenes detection as fast, easy, and reliable as possible.

For testing of Food and Environmental samples only.
For testing of Food and Environmental samples only.

Specifications

Target Organism Class: L. monocytogenes
Validated Application: Real-Time PCR
Detection Method: Primer-Probe Detection
PCR Method: qPCR
For Use With (Equipment): 7500 Fast System, StepOnePlus™, Fast Mode, StepOne™, Fast Mode, MagMAX™ Express-96 Deep Well Magnetic Particle Processor
Sample Type (Specific): Food, Any Swab Type, Water Samples
Product Size: 96 assays

Contents & storage

Detection Kit
• 12 8-tube strips containing assay beads (store at 2 to 8 °C)
• Optical 8-tube strips and 8-cap strips (store at room temperature)
• Negative Control (store at 2 to 8 °C)
• Protocol and Quick Reference Card
The detection kit is sufficient for 96 assays.

Extraction Kit
• 2 bottles of Lysis Buffer (store at room temperature)
• 2 tubes of Magnetic Particles (store at 2 to 8 °C)
• 1 empty bottle for binding solution (store at room temperature)
• 1 bottle of Wash Buffer Concentrate (store at room temperature)
• 1 bottle of Elution Buffer (store at room temperature)
• 1 bottle of Proteinase K Buffer (store at room temperature)
• 1 tube of Proteinase K (store at -20 °C)
The extraction kit is sufficient for 100 extractions.

Documents

Manuals & protocols