PrepSEQ™ Residual DNA Sample Preparation Kit

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures ofWeitere Informationen

Katalognummer	Menge
4413686	100 Präp.

Katalognummer 4413686

Preis (EUR)

1.108,00

Zum Warenkorb hinzufügen

Menge:

100 Präp.

Preis (EUR)

1.108,00

Zum Warenkorb hinzufügen

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures of proteins, buffers, and salts which are typical of samples that need to be quantitated for residual host cell line DNA. Quantitative and consistent recovery of DNA can be obtained from challenging matrices, including samples that have high protein concentration, low pH, or high salt.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

EndprodukttypGenomische DNA

Zur Verwendung mit (Anwendung)Impurity Testing

Hochdurchsatz-KompatibilitätNot High-throughput Compatible (Manual), Automated Protocols

Anzahl Reaktionen100 Aufreinigungen

ProduktliniePrepSEQ

Menge100 Präp.

ProbentypBiopharmaceutical Production Samples

Probenvolumen100 μl

UmfangMini

ZielGenomische DNA

Prüfzeit2 hr.

Isolation TechnologyMagnetic Bead

Unit SizeEach

Inhalt und Lagerung

Includes buffers, magnetic beads and enzymes for DNA extraction for residual DNA quantitation for 100 reactions

Häufig gestellte Fragen (FAQ)

The magnetic beads, that are shipped with the PrepSeq Residual DNA Sample Preparation Kit (Cat. No. 4413686), are clumped together and are difficult to get back into suspension. How can I fix this?

The beads are known to clump due to precipitation of the salts in the solution. These are highly concentrated chaotropic salt solutions. The only way to get the salts and the beads back into the solution is by incubating them at 37 degrees C for at least 10 minutes or until the solution is homogenous. Intermittent high speed vortexing helps expedite the process. To get beads stuck to the lid back into solution, we recommend inverting the tube and using high speed vortexing in a pulsing manner.
If incubating at 37 degrees C does not fix the issue, we recommend incubating at 45 degrees C.

What amount of DNA can be recovered with the PrepSEQ Residual DNA Sample Preparation Kit (Cat. No. 4413686)?

With the above kit, up to 5 ng of DNA can be recovered per 30 µl magnetic beads used per reaction. PrepSEQ is specially designed to recover lower amounts of DNA (in ng to sub femto‐gram levels).