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MicroSEQ® Salmonella spp. Detection Starter Kit with PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean

Catalog number: 4415044

Applied Biosystems™  Related applications: Diagnostic Testing | Food & Beverage

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The MicroSEQ® Salmonella spp. Detection Kit Starter Kit with PrepSEQ™ RapidSpin Sample Preparation Kit – Extra Clean enables the detection of Salmonella spp. simply, reliably, and rapidly in food samples using the Applied Biosystems® 7500 Fast Real-Time PCR System. It is recommended for first time users in laboratories that routinely prepare up to 100 samples a day and prefer a spin column-based workflow. This starter kit combines the PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean with a MicroSEQ Salmonella spp. Detection Kit for ease of ordering. The benefits of this workflow include:

• Enjoy the ease-of-use of a streamlined, lypholized format and simple, software-guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 19 hours total)
• Detect 1-3 colony-forming units (CFUs) per 25 grams of food
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with an internal positive control
• Validated by the AOAC Research Institute

Simple, Three-step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps:
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean is optimized for use with the MicroSEQ Salmonella spp. Detection Kit. The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean reduces pre-enrichment time, enhances assay performance, and enables you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 CFUs per 25 grams of sample. The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean includes extra tubes for a transfer step, which is recommended for foods with a high lipid content, such as infant formula and chicken wing samples. However, simpler matrices can also be prepared with this kit by skipping the transfer step.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ Salmonella spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Clear Results Using a Software-guided Procedure
After sample enrichment and preparation, RapidFinder™ Express Software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection, and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications, and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow including the MicroSEQ Salmonella spp. Detection Kit, PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean, Applied Biosystems 7500 Fast Real-time PCR System, and RapidFinder Express Software has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ Salmonella spp. Detection Kit with PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Salmonella detection as fast, easy and reliable as possible.
For Testing of Food and Environmental Samples Only.


Target Organism Class: Salmonella spp.
Validated Application: Real-Time PCR
Detection Method: Primer-Probe Detection
PCR Method: qPCR
For Use With (Equipment): 7500 Fast System, StepOne™, Fast Mode, StepOnePlus™, Centrifuge, StepOnePlus™, Fast Mode
Sample Type (General): Bacteria, Food & Environmental Samples
Sample Type (Specific): Food, Food (High Lipid Content), Water Samples, Bacteria (Hard-to-Lyse), Any Swab Type
Hot Start: Built-In Hot Start
Reaction Speed: Fast
Passive Reference Dye: ROX (Pre-mixed)
Primer-Probe Compatibility: TaqMan®
Polymerase: AmpliTaq® Fast DNA Polymerase
Product Size: 1 kit
Number of Reactions: 96 Tests

Contents & storage

This kit contains 8-tube strips with assay beads (store at 2 to 8°C), Optical 8-tube strips and 8-cap strips (store at room temperature), a Negative Control (store at 2 to 8°C), Spin columns, Microcentrifuge tubes and Lysis Buffer (store at 4°C).

Protocols and Quick Reference Cards are included. The kit is sufficient for 96 assays.


Manuals & protocols