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PrepSEQ® Rapid Spin Sample Preparation Kit with Proteinase K

Catalog number: 4426714

Applied Biosystems™  Related applications: DNA Extraction | Diagnostic Testing

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The PrepSEQ® Rapid Spin Sample Preparation Kit with Proteinase K is recommended for difficult to lyse bacteria like Listeria monocytogenes. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.

For Research Use Only. Not for use in diagnostics procedures.
For Testing of Food and Environmental Samples Only.


Sample Type (General): Liquid Samples (e.g. Serum), Food & Environmental Samples, Bacteria
Sample Type (Specific): Bacterial Culture, Bacteria (Hard-to-Lyse)
Isolation Technology: Spin Column, Enzymatic, Coarse Filtration
Downstream Application: PCR, Real-Time Quantitative PCR (qPCR)
Final Product: Bacterial DNA
For Use With (Equipment): Centrifuge
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Format: Spin Column
Product Size: 1 kit
Number of Reactions: 100 reactions
Starting Material (Amount): 750 µl

Contents & storage

Each kit contains 100 spin columns, 100 microcentrifuge tubes, Lysis Buffer (store at 4°C), and Proteinase K (store at -20°C). 100 samples can be prepared using this kit.


Manuals & protocols