MicroSEQ® ID Purification Combo Kit, with clean-up cartridges

Catalog number: 4427807

Applied Biosystems™  Related applications: Microbial Identification & Detection | Microbial Protein Production

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Description

This MicroSEQ® ID Purification Combo Kit includes reagents for post-PCR and post-sequencing steps in the MicroSEQ® ID microbial identification workflow. It contains 2 tubes of ExoSAP-IT® Reagent for PCR clean-up and 36 MicroSEQ® ID Sequencing Clean-up Cartridges.

ExoSAP-IT® Reagent: this reagent is used for quick post-PCR cleanup for downstream applications such as sequencing. It allows for removal of unconsumed dNTPs and primers remaining in the PCR mix following amplification.

ExoSAP-IT® Reagent can be used downstream of the following PCR amplification kits:
• MicroSEQ® 500 16S rDNA PCR Kit
• MicroSEQ® Full Gene 16S rDNA PCR Kit
• MicroSEQ® D2 rDNA Fungal PCR Kit

MicroSEQ® ID Sequencing Clean-up Cartridges: these cartridges are used for post-sequencing clean-up in the MicroSEQ® ID workflow. The gel filtration matrix used in the cartridges provide a convenient and simple way to remove dye terminators, dNTPs, and salts, as well as residual primers from cycle sequencing reactions.

The MicroSEQ® ID Sequencing Cleanup Cartridges can be used downstream of the following sequencing kits:
• MicroSEQ® 500 16S rDNA Sequencing Kit
• MicroSEQ® Full Gene 16S rDNA Sequencing Kit
• MicroSEQ® D2 rDNA Fungal Sequencing Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Spin Column
System: MicroSEQ® ID
Product Size: 1 kit
Final Product: DNA
Number of Reactions: 36 Preps
Isolation Technology: Spin Column, Enzymatic
Sample Type (General): dsDNA
Downstream Application: Sequencing
Sample Type (Specific): Double-Stranded DNA (dsDNA)

Contents & storage

The kit contains an Exo SAP-IT PCR Purification kit and DTR Ultra Cartridge.

Documents

Manuals & protocols