3500 Genetic Analyzer for Resequencing & Fragment Analysis

Catalog number: 4440462

Applied Biosystems™  Related applications: DNA Sequencing | Fragment Analysis | Genotyping & Genomic Profiling | RNA Sequencing | Sanger Sequencing

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Description

The 3500 Genetic Analyzer is an 8-capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process controlled environments, while retaining the unsurpassed application versatility that life science researchers expect.

This package includes an 8-capillary instrument, 3500 Series Data Collection Software, a Dell Workstation and monitor, as well as reagent kits for system qualification and secondary analysis software. The 3500 Series Data Collection Software supports sequencing and fragment analysis, and integrates seamlessly with downstream software for secondary analysis of genetic data. This package includes such secondary software: GeneMapper® software for fragment analysis and software for resequencing applications.

Features include:

• An 8 capillary system that can easily be upgraded to a 24-capillary system when you’re ready.
• Single-line, 505 nm, solid-state, long-life laser that utilizes a standard power supply and requires no heat-removal ducting
• Powerful, integrated data collection and primary analysis software provides real-time assessment of data quality
• Radio frequency identification (RFID) technology tracks key consumables data and records administrative information
• Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes
• Unrivaled application flexibility—one array and one polymer are used for most applications.
• Simple setup, operation and maintenance

Accurate Reliable Data Quality
The 3500 series systems significantly improves signal uniformity from instrument-to-instrument, run-to-run, and capillary-to-capillary. With a combination of intelligent hardware, powerful new algorithm and reagent combinations, these methods provide major reduction in the range of signal peak heights obtained across multiple 3500 series instruments (see figure).

Improved Sizing Precision
New thermal sub-system design gives enhanced performance in demanding fragment applications enabling improved sizing precision versus previous generations of capillary electrophoresis platforms.

Ready to Use Consumables With RFID Tags
The 3500 series introduces ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers, and easy-to-install capillary arrays. Each of these consumables is integrated with RFID tags that enable viewing, tracking, and reporting of critical information about reagents and consumables including usage, lot number, part number, expiry date, and on-instrument lifetime within the 3500 Series Data Collection Software.

Easy to Use Data Collection Software
3500 Series Data Collection software breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables and handy maintenance scheduling calendar functionality. New functionality includes simplified plate set-up and built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced on the instrument without the need to transfer output files into secondary analysis software packages.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 8-well strips, 96-well plate
Weight: 82 kg (approximately)
Throughput: Up to 424 samples per day (using run module HID36 POP-7)
Read Length: >850 bp using a 50 cm capillary and POP-7™ polymer
Product Size: 1 system
Current Limit: 15 amps
Plan Duration: 1 Year Limited Warranty on Parts and Labor, Service Installation and Application Training Included
Voltage Limit: Electrophoresis Voltage - Up to 20 kV
Capillary Length: 36 cm, 50 cm
Operating System: Windows 7 (Service Pack 1)
Analysis Software: Sequencing Analysis Software, GeneMapper® Software, Variant Reporter® Software
External Computer: Memory: 16 GB (2x 8GB) 1600 MHz DDR3 Non-ECC. 4th Gen Intel Core I7 Processor- 3.1 GHz Turbo Processor, Operating System: Windows 7 SP1, Hard Drive: 2x 500GB SATA 3.0 Gb/s and 8 MB Data Burst Cache
Compatible Polymers: POP-4™, POP-6™, POP-7™
Compatible Products: 3500 Genetic Analyzer Capillary Array (50 cm), POP-6™ Polymer for 3500⁄3500xL Genetic Analyzers, 3500 Genetic Analyzer Capillary Array (36 cm), POP-4™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-7™ Polymer for 3500⁄3500xL Genetic Analyzers
Polymer Consumption: Replace at earliest of 14 days⁄96 samples⁄12 injections (96-sample pouches), Replace at earliest of 14 days⁄960 samples⁄120 Injections (960-sample pouches), Replace at earliest of 14 days⁄384 samples⁄60 Injections (384-sample pouches)
Number of Capillaries: 8 Capillaries
Operating Environment: Room temp: <2° fluctuation during runs, Temperature: 15°C-30°C, Humidity: 20%-80% (non-condensing)
For Use With (Equipment): 3500 Genetic Analyzer

Contents & storage

This package consists of the 8 capillary instrument, the 3500 Data Collection Software, a Dell Workstation and monitor as well as reagent kits for system qualification. This package also includes GeneMapper® software for fragment analysis and software for resequencing applications.

Documents

Manuals & protocols