TaqMan® Protein Assays Core Reagents Kit with MasterMix

Catalog number: 4448591

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Stem Cell Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The TaqMan® Protein Assays Core Reagents Kit with MasterMix contains both the Core Reagents Base Kit and the TaqMan Protein Assays Fast Master Mix Kit. The Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan® Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan® Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Hot Start: No
Technique: Proximity Ligation Assay
PCR Method: qPCR Protein Quantification
Polymerase: AmpliTaq® Fast DNA Polymerase
Label or Dye: FAM™
Product Size: 500 reactions
Reaction Speed: Fast
Detection Method: Primer-Probe Detection
Target Specificity: Not Target-Specific
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (General): Cells, Tissue
For Use With (Equipment): 7900HT System, 7500 System, 7900HT Fast System, StepOnePlus™, Fast Mode, 7500 Fast System
Primer-Probe Compatibility: TaqMan®

Contents & storage

The TaqMan® Protein Assays Core Reagents Kit with MasterMix contains both the Core Reagents Base Kit and the TaqMan Protein Assays Fast Master Mix Kit.

Documents

Manuals & protocols