Applied Biosystems™

TaqMan™ Protein Assays Core Reagents Base Kit

Catalog number:  4448592

 Related applications: Real Time PCR (qPCR) | Stem Cell Analysis

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TaqMan® Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode
Hot Start: No
Label or Dye: FAM™
PCR Method: qPCR Protein Quantification
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq® Fast DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqMan™
Product Size: 500 reactions
Reaction Speed: Fast
Sample Type (General): Cells, Tissue
Target Specificity: Not Target-Specific
Technique: Proximity Ligation Assay
Shipping Condition: Wet Ice

Contents & storage

The TaqMan® Protein Assays Core Reagents Base Kit contains:

DNA Ligase, 500X (1 × 50 µL)
Ligase Dilution Buffer, 1X (1 × 15 mL)
Ligation Reaction Buffer, 20X (1 × 4 mL)
Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 5 mL)
Protease, 100X (1 × 50 µL)
Universal PCR Assay, 20X (1 × 600 µL)
Store all components at -15 to -25°C.


Manuals & protocols