MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes

Catalog number: 4451893

Ambion™  Related applications: RNA Extraction

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Description

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from Tempus™ Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.

• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
• Simple magnetic bead workflow—no spin columns
• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors

Maximize Total RNA Yields From Blood Samples
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from Tempus™ Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-25 µg from a single Tempus™ Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from Tempus™ Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.

Flexible Purification Formats
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods.
• For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step.
• For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process.
• For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).

Rapid Processing Time
Processing times will vary based on format selected, but twelve Tempus™ Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.

An Alternative to Full Purification
For users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from Tempus™ Blood RNA tubes without conventional RNA purification.

One-Stop Shopping
MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Yield (Range): 3 - 25µg
• Quality (RIN) ): ≥ 7
• Quality (A260⁄280) ): 1.8 – 2.2
• gDNA (w⁄w) ): <1.56%
• Equipment Needed :
     *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with
     *50 mL conical tube adapters
     *50ml conical tubes
     *For tube format users: Tabletop microcentrifuge or picocentrifuge
• Number of Preps: 96 preparations (blood tubes)

NOTE: Blood tubes and magnetic stands are sold separately.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
High Throughput Compatibility: Automated Protocols, Not High Throughput-Compatible (Manual), High Throughput-Compatible
Downstream Application: Real-Time Quantitative PCR (qPCR)
Format: Tube(s), 96-well plate
Product Size: 96 preps
Final Product: Total RNA
Yield (up to): 25 µg
Purification Time: 1.9 hrs
Isolation Technology: Magnetic Bead

Contents & storage

Kit Components
Box 1:
Store at RT

• Tempus® Resuspension Solution, 12 mL
• Binding Solution Concentrate 5 mL
• Wash Solution 1 Concentrate, 2 x 16mL (Before using the Wash Solution 1 Concentrate, add the correct volume of isopropanol (user-supplied), as indicated on the bottle)
• Wash Solution 2 Concentrate, 30 mL (Before using the Wash Solution 2 Concentrate, add the correct volume of ethanol (user-supplied), as indicated on the bottle)
• Elution Buffer 10 mL
• Processing Plate (96-well) 1 plate

Store at 2 to 8 °C, Do not freeze.
• Tempus® Pre-Digestion Wash, 2 x 200 mL
• RNA Binding Beads, 2 mL
• Tempus® 1X PBS, 2 x 150 mL

Box 2:
Store at -15 to -25 °C
• Tempus® Proteinase, 0.25 mL
• TURBO™ DNase, 1 mL

Documents

Manuals & protocols