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Invitrogen™

MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes

Catalog number: 4451893
Invitrogen™

MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes

Catalog number: 4451893
Catalog Number
4451893
Unit Size
96 preps
Price (USD)
Price: 886.00
Online price: 874.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
445189396 preps
Price: 886.00
Online price: 874.65
Your price:
-
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The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from Tempus™ Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.

• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
• Simple magnetic bead workflow—no spin columns
• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors

Maximize Total RNA Yields From Blood Samples
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from Tempus™ Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-25 μg from a single Tempus™ Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from Tempus™ Blood RNA tubes as shown by TaqMan™ gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.

Flexible Purification Formats
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods.
• For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step.
• For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process.
• For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).

Rapid Processing Time
Processing times will vary based on format selected, but twelve Tempus™ Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.

An Alternative to Full Purification
For users who perform primarily TaqMan™-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from Tempus™ Blood RNA tubes without conventional RNA purification.

One-Stop Shopping
MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript™), PCR master mixes (both TaqMan™ and SYBR™ based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Yield (Range): 3 – 25μg
• Quality (RIN) ): ≥ 7
• Quality (A260⁄280) ): 1.8 – 2.2
• gDNA (w⁄w) ): less than 1.56%
• Equipment Needed :
   *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with
   *50 mL conical tube adapters
   *50ml conical tubes
   *For tube format users: Tabletop microcentrifuge or picocentrifuge
• Number of Preps: 96 preparations (blood tubes)

NOTE: Blood tubes and magnetic stands are sold separately.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Magnetic Bead
Purification Time
Less than 2 hr. for automated protocol
Elution Volume
20 to 80 μL
Sample Type
Whole Blood
Final Product Type
Total RNA
High-throughput Compatibility
High-throughput Compatible
For Use With (Equipment)
KingFisher™ Duo Prime, KingFisher™ Flex
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity
96 Preps
Shipping Condition
Box 1: Room Temperature
Box 2: Cold Gel
Starting Material Amount
3 mL of whole blood collected in Tempus™ Blood RNA Tube
Yield
≤25 μg

Contents & Storage

Box 1
• 12 mL Tempus Resuspension Solution; room temperature
• 5 mL Binding Solution (concentrated); room temperature
• 2 x 16 mL Wash Solution 1 (concentrated); room temperature
• 30 mL Wash Solution 2 (concentrated); room temperature
• 10 mL Elution Buffer; room temperature
• 1 Processing Plate; room temperature
• 2 x 200 mL Tempus Pre-Digestion Wash; 4°C
• 2 mL RNA Binding Beads; 4°C
• 2 x 150 mL Tempus 1X PBS; 4°C

Box 2
• 0.25 mL Tempus Proteinase; -20°C
• 1 mL TURBO DNase; -20°C

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