Single Cell Lysis Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 4458235

Ambion™  Related applications: RNA Extraction | Real Time PCR (qPCR) | Viral RNA⁄DNA Purification

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Description

Single cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge. The Ambion® Single Cell Lysis Kit enables reliable RNA preparation for single cells.

Key product features:

• Optimized sample prep—Enables RNA preparation for single cells
• Efficient design—No sample loss ensures maximal sensitivity
• Versatile application—Compatible with common enzymatic reactions

Optimized RNA Sample Prep for 1-10 Cells
The Ambion® Single Cell Lysis Kit enables RNA preparation from 1-10 cells. The Single Cell Lysis kit generates a concentrated cell lysate through the optimized combination of lysis buffer, stop solution, and DNase. The resulting lysate is compatible with common enzymatic reactions such as reverse transcription. Sample preparation is fast and can be completed in only 7 minutes with 2 pipetting steps (Figure 1).

No Sample Loss Ensures Maximal Sensitivity
The Ambion® Single Cell Lysis Kit bypasses the need for traditional RNA purification. There are no sample transfers, columns or washes which can lead to loss and⁄or dilution of the precious sample. The entire lysate can be used in downstream reactions ensuring maximal sensitivity and accuracy in your experiments.

Compatible with Common Enzymatic Reactions
The reagents in the Ambion® Single Cell Lysis Kit are identical to the lysis reagents in the complete Single Cell-to-CT™ Kit for Real-time RT-PCR analysis from 1-10 cells. They have been validated with multiple reverse transcription kits including SuperScript® VILO™ cDNA Synthesis Kit, High Capacity RNA-to-cDNA kit and TaqMan® MicroRNA Reverse Transcription Kit, for analysis of mRNA as well as miRNA targets. As a stand alone module, the Single Cell Lysis Kit allows you to create new workflows to expand the possibilities of single cell analysis.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Sample Type (General): Bacteria, Viral Samples, Tissue, Blood, Cells
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Product Size: 100 preps
Final Product: Lysate
Starting Material (Amount): 1-10 cells
Green Features: Fewer resources used, Less hazardous, Less waste
Shipping Condition: Dry Ice

Contents & storage

Kit contains:
  • 100 µl Single Cell Dnase I: -20°C;
  • 1 ml Single Cell Lysis Solution: 4°C;
  • 100 µl Single Cell Stop Solution: -20°C

Documents

Manuals & protocols