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MagMAX™ FFPE Total Nucleic Acid Isolation Kit - FAQs

View additional product information for MagMAX™ FFPE Total Nucleic Acid Isolation Kit - FAQs (4463365)

8 product FAQs found

Can the buffers of the MagMAX FFPE DNA/RNA Ultra Kit be used for the MagMAX FFPE Total Nucleic Acid Isolation Kit and vice versa?

No, the buffers of the two kits are quite different and cannot be used interchangeably.

I can see on your website that you offer several MagMAX kits for FFPE samples. Which kit do you generally recommend?

We recommend using the MagMAX FFPE DNA/RNA Ultra Kit since it is the most versatile and optimized kit. In addition to just isolating RNA or DNA, it allows for sequential isolation of RNA and DNA from the same FFPE sample.

What is the major difference between the MagMAX FFPE Total Nucleic Acid Isolation Kit and MagMAX FFPE DNA/RNA Ultra Kit?

The MagMAX FFPE DNA/RNA Ultra Kit allows for the sequential isolation of RNA and DNA from the same FFPE sample. The MagMAX FFPE Total Nucleic AcidIaolation kit requires you to perform separate protocols for RNA and DNA extraction using separate FFPE sections.

Can I edit the manufactured KingFisher Flex protocols?

No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.

Can I use magnetic beads from other vendors with the KingFisher Flex instrument?

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I am working with liquid samples with high volume and want to extract nucleic acid from them using the KingFisher Flex instrument. Can I use more volume than the protocol advises?

Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Can I scale up my reaction volume with the KingFisher Flex instrument?

Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.

Are all of the Applied Biosystems MagMAX kits validated for the KingFisher Flex Magnetic Particle Processor?

Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.