Platinum™ Multiplex PCR Master Mix, 50 reactions - FAQs

Yes, the Platinum Multiplex PCR master mix contains Platinum Taq DNA Polymerase that leaves A overhangs on PCR products.

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Yes, Platinum Multiplex PCR Master Mix contains Platinum Taq Polymerase that can incorporate dUTP and amplify uracil-containing templates.