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Platinum™ Multiplex PCR Master Mix, 250 reactions - FAQs

View additional product information for Platinum™ Multiplex PCR Master Mix - FAQs (4464269, 4464268, 4464270)

3 product FAQs found

Does the Platinum Multiplex PCR master mix leave A overhangs?

Yes, the Platinum Multiplex PCR master mix contains Platinum Taq DNA Polymerase that leaves A overhangs on PCR products.

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Can I use Platinum Multiplex PCR Master Mix for amplifying uracil-containing templates?

Yes, Platinum Multiplex PCR Master Mix contains Platinum Taq Polymerase that can incorporate dUTP and amplify uracil-containing templates.