Applied Biosystems™

SOLiD™ EZ Bead™ Emulsifier E120 Reagents Kit

Catalog number:  4465555

 Related applications:


| SOLiD® Next-Generation Sequencing

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The SOLiD™ EZ Bead™ Emulsifier combines an aqueous phase with an oil phase, creating an emulsion that contains microreactors containing the necessary reagents and components for amplifying the library of interest onto a solid surface, namely beads. This process takes up to 30 minutes to complete, depending on the desired bead yield.

The new SOLiD™ EZ Bead™ System simplifies next-generation sequencing workflows by providing an automated solution for reproducible templated bead preparation with less than 1 hour of hands-on time. The system is specifically designed with system modularity and quality control check points for greater experimental control. SOLiD™ EZ Bead™ System streamlines the sequencing workflow through significant time savings and support for up to 96 multiplexed libraries.

• 80% reduction in hands-on time
• Increased reproducibility
• Multiplexed library support, which facilitates maximum time and cost savings
• Modularity to fit individual throughput and lab needs
• Quality control checkpoints throughout the workflow for greater experimental control

Automated Workflow
The SOLiD™ EZ Bead™ System automates theSOLiD™ System workflow from emulsion PCR to templated bead deposition. The availability of pre-packaged reagents minimizes handling errors and improves the quality and quantity of templated beads. With less than 1 hour of hands-on time, sample preparation time is reduced by 80% (Figure 1).

Quality Control
The open architecture of the SOLiD™ EZ Bead™ System offers quality control checkpoints to ensure high quality sequencing results. Self-contained consumables and automated decontamination procedures help minimize cross contamination between samples, further increasing data quality.

Modular Design
Specifically designed to fit individual throughput and laboratory needs, the SOLiD™EZ Bead™ System facilitates customizable instrument configurations to align with your research. The system’s three modules, the SOLiD™ EZ Bead™ Emulsifier, the SOLiD™ EZ Bead™ Amplifier, and the SOLiD™ EZ Bead™ Enricher, support multiple sequencing instruments and extend to the entire family of SOLiD™ Analyzers.

Scalable Throughputs
The SOLiD™ EZ Bead™ System supports bead outputs for 1, 4, and 8-well slide configurations with corresponding E10, E20,and E80 prepackaged reagents to accommodate a variety of high-, medium-,and low-throughput applications. Additional support for multiplexed libraries helps further allows for high quality, cost effective sequencing.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): EZ Bead™ System
High Throughput Compatibility: High Throughput-Compatible
Output: Templated beads
Product Line: EZ Bead™, SOLiD™
Product Size: 1 each
Recommended Applications: De Novo Sequencing, Shotgun Sequencing
Technique: Ligation-Based Sequencing
Workflow Step: Emulsion PCR

Contents & storage

The reagent kits contain all of the buffers and consumables for the SOLiD™ EZ Bead™ Emulsifier. The average yield of templated beads prepared using the E120 kit is 2.2 billion.


Manuals & protocols