Thermo Scientific™

POROS™ CaptureSelect™ HSA Affinity Column, 4.6 x 100 mm

Catalog number: 4469170
Thermo Scientific™

POROS™ CaptureSelect™ HSA Affinity Column, 4.6 x 100 mm

Catalog number: 4469170
Catalog Number
4469170
Unit Size
1 column
Price (USD)
3,400.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44691701 column
3,400.00
Your Price:
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Product Overview
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Additional Information
Recommendations
Recommendations
Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

Poros™ CaptureSelect™ HSA HPLC columns incorporate the advantages of high-speed Poros chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three minute quantitation of HSA fusion proteins.

Features:

• Broad utility—POROS™ A, G and CaptureSelect™affinity ligands address most biological drug classes
• Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—deliver high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ HSA affinity ligands when combined with POROS™ 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of HSA Fusion proteins.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.

Automation
POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS™ Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

HSA Column Specifications:
Protein Binding Capacity 2.6 mg/mL
Species Specifiity Human
Column Material PEEK™ polymer or stainless steel
Frit 2 µm, titanium
POROS™ Resin Cross-linked poly (styrenedivinylbenzene)
CaptureSelect™ Ligands Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae


For Research Use Only. Not for use in diagnostics procedures.

Specifications

Quantity
1 Column
Stationary Phase
CaptureSelect™ HSA
Column Type
Affinity, Prepacked
Form
Column
Product Line
CaptureSelect™, POROS™
Target
HSA, HSA Fusion Proteins
Type
Affinity Column
Capacity (Metric)
1.7 mL Column Volume
Flow Rate
Up to 5000 cm/hr.
Matrix
Polystyrenedivinylbenzene
Pore Size
0.16 micron
Binding Strength
2.6 mg/ml dynamic binding capacity
Elution Volume Max
3.4 ml
Elution Volume Min
1.7 ml
For Use With (Equipment)
HPLC
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Max. Operating Pressure
180 bar
Shipping Condition
Wet Ice
Reactivity
Human
Sample Type
Cell Culture Supernatants
Particle Size
20 micron

Contents & Storage

Column includes EZ grip fittings and column test certificate



Store at 4°C DO NOT FREEZE

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