QuantStudio™ Digital PCR Kit

Catalog number: 4470184

Applied Biosystems™  Related applications: Digital PCR | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

QuantStudio™ Digital PCR Kits enable the accurate and sensitive quantitation of materials beyond the limits of conventional PCR. The kits include QuantStudio™ Digital PCR Plates and 2X TaqMan® Digital Master Mix. This 10-plate size also includes a QuantStudio™ 12K Flex OpenArray® Accessories Kit. The QuantStudio™ Digital PCR Kits are intended for use with our QuantStudio™ 12K Flex System, which enables over 12,000 digital PCR reactions to be run simultaneously to generate up to 48,000+ digital data points per day.

Key Product Features:

• Accurate and sensitive—Detect and count individual molecules to quantify viral load, gDNA, cDNA, plasmids, or next generation sequencing libraries
• Fast—produce up to 192 digital answers per ~2 hour run
• Flexible—Use your existing assays to test from one to 48 assay/sample dilutions per plate
• Wide dynamic range—By accommodating as few as 64 data points per replicate group, a dilution series can easily be loaded into the QuantStudio™ Digital PCR Plate, greatly expanding the range of sample concentrations that can be analyzed to produce a digital answer
• Intuitive and economical—Meet the needs of both your qPCR and dPCR studies on a single unified instrument platform

Simple Workflow
QuantStudio™ Digital PCR Plates are delivered pre-treated with hydrophilic and hydrophobic coatings to accept your own TaqMan® assays. Simply combine the assays with sample and master mix, load onto the QuantStudio™ plate, seal, cycle, and image. The QuantStudio® 12K Flex System's simple workflow enables one person to generate over 48,000 digital data points in one day without the use of additional robotics.

Flexible Formats
Each QuantStudio™ Digital PCR Plate contains 3,072 through-holes arranged in 48 subarrays of 64 through-holes each. The OpenArray® AccuFill™ System and open plate format enables the loading of anywhere between one and 48 samples or sample dilutions per plate dependent upon experimental needs.

A Complete Digital PCR Solution
The QuantStudio™ Digital PCR solution is an affordable, whole-system solution for numerous Digital PCR applications. The decrease in reagent use afforded by this unique combination of technologies greatly decreases the price per data point, allowing more samples to be run for a lower cost of consumables. QuantStudio™ Digital PCR Plates, TaqMan® Digital PCR Master Mix, QuantStudio™ accessories, and DigitalSuite™ PCR Software have been designed to work together to enable you to obtain accurate and reproducible Digital PCR results.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): QuantStudio™ 12k Flex
Form: Frozen
Format: OpenArray® Plate
Hot Start: Built-In Hot Start
Label or Dye: FAM™
Number of Reactions: 30,720 Reactions
PCR Method: Digital PCR
Passive Reference Dye: ROX (Pre-mixed)
Primer-Probe Compatibility: TaqMan®
Product Line: QuantStudio™
Product Size: 10 plates
Reaction Speed: Standard
Sample Type (General): DNA

Contents & storage

10 QuantStudio™ Digital PCR Plates — Store at -20°C



2X TaqMan® Digital Master Mix — Store at -20°C until first use, then 4°C thereafter



QuantStudio™ 12K Flex OpenArray® Accessories Kit — Store at room temperature

Documents

Manuals & protocols

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