AmpFLSTR® NGM SElect™ Express Kit

Catalog number: 4472193

Applied Biosystems™  Related applications: PCR Amplification for Human Identification

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Description

The AmpFℓSTR® NGM SElect™ Express Kit enables extremely efficient analysis of single-source samples for laboratories wishing to utilize the European Standard Set Loci (ESSL) together with the highly discriminating SE33 locus. The kit utilizes the same primer sequences as the NGM™ and NGM SElect™ kits, but employs a new master mix optimized to enable high quality direct amplification of swab and treated/untreated paper substrates, as well as rapid PCR cycling (< 1 hr).

Key Features of the AmpFℓSTR® NGM SElect™ Express Kit:

• Supports rapid, direct amplification on treated paper and untreated paper/swab substrates in conjunction with Prep-n-Go™ Buffer
     - Facilitates high throughput & automated processing
     - Minimizes extraction, purification, and quantification costs and helps reduce labor and consumable costs
• Contains a new fast-capable enzyme that allows completion of amplification in ~45 mins
• Maximizes first pass success rate, reducing the need for time-consuming repeat amplifications
• Part of our complete integrated solution for efficient single-source sample processing
• Enables laboratories to go from sample to result typically in only 2 hours!

Data Quality Worth Sharing
The AmpFℓSTR® NGM SElect™ Express Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) and includes the expanded European Standard Set of Loci (ESSL). The NGM SElect™ Express kit simultaneously amplifies the same 16 loci included in the NGM™ kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel.

Powerful Discrimination
The combination of loci in the NGM SElect™ Express kit generates an extremely high power of discrimination of any AmpFℓSTR® kit (see Table 1).

Maximizes Concordance
The NGM SElect™ Express kit maintains identical primer sequences for the core STR loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ kits, thereby:

• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements

The primers for the SE33 locus were redesigned from those used in the SEfiler Plus™ kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus™ kit.

Easy to Implement, Easy to Use
Every aspect of the NGM SElect™ Express kit has been designed with ease of implementation and use in mind. This is a single kit solution that is designed to deliver high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs. The kit workflow is very similar to other AmpFℓSTR® kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened to less than one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.

For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For Forensic or Paternity Use Only.

Specifications

Technique: STR (Short Tandem Repeat) Analysis
Label or Dye: NED™, PET®, VIC®, LIZ®, FAM™
Product Size: 200 reactions
Sample Type (Specific): Blood, Buccal Swab, Forensic Sample
For Use With (Equipment): GeneAmp® 9700, Veriti® Thermal Cycler
Matrix and Size Standards: Matrix Standard Set DS-33
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols

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