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Ion Xpress™ Barcode Adapters 17-32 Kit

Catalog number: 4474009

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

The Ion Xpress™ Barcode Adapters 17-32 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.

Key Product Features:

• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Specifications

For Use With (Equipment): Ion OneTouch™ System, Ion PGM™ System
Library Type: Fragment Library
Workflow Step: Library Barcoding
Starting Material: DNA
Sequencing Type: Epigenetic Sequencing, Genome & DNA Sequencing
High Throughput Compatibility: High Throughput-Compatible, Multiplexing
Multiplex Capability: Up to 32 fragment library samples
Product Size: 1 kit
Shipping Condition: Dry Ice

Contents & storage

Ion Xpress™ P1 Adapter, 1 tube, 320 µL, –30 to –10°C
Ion Xpress™ Barcode 17, 1 tube, 20 µL
Ion Xpress™ Barcode 18, 1 tube, 20 µL
Ion Xpress™ Barcode 19, 1 tube, 20 µL
Ion Xpress™ Barcode 20, 1 tube, 20 µL
Ion Xpress™ Barcode 21, 1 tube, 20 µL
Ion Xpress™ Barcode 22, 1 tube. 20 µL
Ion Xpress™ Barcode 23, 1 tube, 20 µL
Ion Xpress™ Barcode 24, 1 tube, 20 µL
Ion Xpress™ Barcode 25, 1 tube, 20 µL
Ion Xpress™ Barcode 26,1 tube, 20 µL
Ion Xpress™ Barcode 27, 1 tube, 20 µL
Ion Xpress™ Barcode 28, 1 tube, 20 µL
Ion Xpress™ Barcode 29, 1 tube, 20 µL
Ion Xpress™ Barcode 30, 1 tube, 20 µL
Ion Xpress™ Barcode 31, 1 tube, 20 µL
Ion Xpress™ Barcode 32, 1 tube,20 µL

Documents

Manuals & protocols