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Ion AmpliSeq™ Library Kit 2.0

Catalog number: 4475345

Ion Torrent™  Related applications: DNA Sequencing | Ion Torrent™ Next-Generation Sequencing

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The Ion AmpliSeq™Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ready-to-use panels and custom primer pools, for sequencing on the Ion Personal Genome Machine™ (PGM™) System.

Key Features:

New Simple Protocol
• 96-well plate-based workflow allows easy sample management
• Only 1 required cleanup step reduces hands-on-time
• No library amplification with qPCR quantification

New Ion AmpliSeq™HiFi Master Mix
• Enables cleaner amplification for increased coverage uniformity
• Up to 3,072-plex PCR allows larger panel designs

New Digestion Reagent
• Cleaner digest allows for more efficient downstream sequencing

Scalable Multiplex PCR Reactions
The Ion AmpliSeq™2.0 technology enables scalable multiplex PCR reactions from 12- to 3,072-plex in a single well using just 10 ng of starting DNA. Ion AmpliSeq™panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Ion AmpliSeq™Custom Primer Pools are designed via the Ion AmpliSeq™Designer web-hosted software, available at

Barcoded Library Preparation
The Ion AmpliSeq™Library Kit 2.0 includes reagents for generating amplicons with Ion AmpliSeq™primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™Barcode Adapters 1-96 kits or IonCode™ Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and Flexible Protocol
The Ion AmpliSeq™Library Kit 2.0 uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The library preparation is completed in as little as 3.5 hours using 10 ng of DNA per PCR, and DNA from a variety of sources—including formalin-fixed, paraffin-embedded (FFPE) tissue can be used as the starting material.

The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™Particles using the automated Ion OneTouch™System. The intuitive Torrent Suite Software and optional Ion Reporter enable you to go from extracted DNA to variant calls in as little as 10 hours.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System
Library Type: Targeted Sequencing Library
Workflow Step: Library Generation
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Technique: Post-Light™ Ion Semiconductor Sequencing, Amplicon Sequencing
Product Size: 8 reactions
Multiplex Capability: 12-plex to 3072-plex per well, 1-96 barcodes
Sample Type (Specific): DNA (Genomic), Tissue (FFPE)
Recommended Applications: Targeted Resequencing
Starting Material (Amount): 10 ng per primer pool

Contents & storage

7 tubes; store at -30 to -10 °C
1 tube Low TE Buffer; store at room temperature


Manuals & protocols

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