Ion AmpliSeq™ Library Kit 2.0

Catalog number: 4475345

Ion Torrent™  Related applications: DNA Sequencing | Ion Torrent™ Next-Generation Sequencing

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Description

The Ion AmpliSeq™ Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ panels.

Scalable Multiplex PCR Reactions
The Ion AmpliSeq 2.0 technology enables scalable multiplex PCR reactions from 12- to 24,000-plex in a single well using just 10 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Ion AmpliSeq™ Custom Primer Pools are designed via the Ion AmpliSeq™ Designer Tool, available at www.ampliseq.com.

Barcoded Library Preparation
The Ion AmpliSeq Library Kit 2.0 includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1-96 kits or IonCode™ Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and Flexible Protocol
The Ion AmpliSeq Library Kit 2.0 uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The library preparation uses very small quantities of starting material per PCR. The resulting DNA libraries are ready for downstream template preparation using the automated Ion Chef™ System (which also provides the option of automated library preparation using the Ion AmpliSeq™ Kit for Chef DL8) or the Ion OneTouch™ 2 System followed by sequencing. The intuitive Torrent Suite™ Software and optional Ion Reporter™ Software enables automation of data analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion Chef™ System, Ion OneTouch™ 2 System, Ion PGM™ System, Ion Proton™ System, Ion S5™ System
Library Type: Targeted Sequencing Library
Workflow Step: Library Generation
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Multiplex Capability: 1-96 barcodes, 12-plex to 3072-plex per well
Product Size: 8 reactions
Recommended Applications: Targeted Resequencing
Sample Type (Specific): DNA (Genomic), Tissue (FFPE)
Starting Material (Amount): 10 ng per primer pool
Technique: Amplicon Sequencing, Post-Light™ Ion Semiconductor Sequencing

Contents & storage

7 tubes; store at -30 to -10 °C
1 tube Low TE Buffer; store at room temperature

Documents

Manuals & protocols

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