Ion Xpress™ RNA-Seq Barcode 1-16 Kit

Ion Xpress™ RNA-Seq Barcode 1-16 Kit - FAQs

View additional product information for Ion Xpress™ RNA-Seq Barcode 1-16 Kit - FAQs (4475485)

3 product FAQs found

Where can I find the sequences for the Ion Xpress RNA-Seq Barcodes 1-16 (Cat. No. 4475485)?

The Ion Xpress RNA-Seq Barcode 1-16 sequences are the same as the DNA library Ion Xpress Barcode Adapter 1-16 sequences and can be found under References in the Torrent Browser.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Why does the Ion Total RNA-Seq Kit v2 (Cat. No. 4475936) specifically require the Ion Xpress RNA-Seq Barcodes 1-16 Kit (Cat. No. 4475485)? Why can't I use the DNA barcodes provided in the Ion Xpress Barcode Adaptors 1-96 Kit (Cat. No. 4474517)?

The Ion Total RNA-Seq Kit v2 workflow uses a different method for adding the barcode sequences to the library than the standard Ion DNA libraries. For standard DNA library construction, the barcoded adapters are directly ligated to the DNA inserts. For Ion Total RNA-Seq libraries, the barcode adapter sequence is added during the final library amplification.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.