Total Exosome Isolation Reagent (from serum) enables fast and efficient enrichment of intact exosomes from blood serum samples. • Maximise recovery of intact exosomes for any type of downstream applications • Easily isolate exosomes with our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size
Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation Reagent (from serum) enables simple and reliable concentration of intact exosomes from blood serum samples with a protocol that is scalable depending on your sample size.
By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the serum sample, and the solution is incubated at 2°C to 8°C for 30 min. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 10 min. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can then be purified using theTotal Exosome RNA and Protein Isolation Kit, or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking. For immunoprecipitation of exosomal proteins, use Exosome Immunoprecipitation (Protein A) or Exosome Immunoprecipitation (Protein G).