AuthentiFiler™ PCR Amplification Kit

Catalog number: 4479566

Applied Biosystems™  Related applications: Genotyping & Genomic Profiling

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The AuthentiFiler™ PCR Amplification Kit is a short tandem-repeat multiplex PCR assay that amplifies 9 unique STR loci and the amelogenin gender-determining marker in a single PCR amplification. This kit is intended for use in human cell identification in various research-based applications requiring human cell discrimination and identity confirmation, such as authentication testing of human cell culture lines and genetic confirmation testing of induced pluripotent stem cells (iPSC).

The AuthentiFiler™ PCR Amplification Kit is:
• An efficient, low-cost PCR amplification kit designed to confirm the genetic identity/source of a human cell
• Performance tested with gDNA extracted from cell pellets, cell culture enrichment media, and formalin-fixed paraffin embedded (FFPE) samples using various gDNA extraction methods and kits
• Highly sensitive—detect low level cross contamination or genetic aborations in your cell lines
• Highly discriminatory, enabling a high level of genetic discrimination between closely related cells, with a small amplicon size that aids in loci recovery from challenging samples (e.g., samples with degraded gDNA, such as FFPE samples)

For Cell Line Authentication Use excluding, Forensic, Paternity, Diagnostic, and Therapeutic applications.


Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, FAM™, VIC®, PET™, LIZ™
Product Size: 50 tests
Amplicon Size: <300 bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
For Use With (Equipment): GeneAmp™ 9700, Veriti® Thermal Cycler, 3500xL Genetic Analyzer, 3130xl Genetic Analyzer, 3130 Genetic Analyzer, 3500 Genetic Analyzer
Matrix and Size Standards: Size Standard: GeneScan&trade 600 LIZ® Size Standard v2.0, Matrix Standard: DS-33 Matrix Standard Kit (Dye Set G5)

Contents & storage

Store at -15 to -25 °C on receipt and 2 to 8 °C after initial use.


Manuals & protocols

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