AuthentiFiler™ PCR Amplification Kit

Catalog number: 4479566

Applied Biosystems™  Related applications: Genotyping & Genomic Profiling

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Description

The AuthentiFiler™ PCR Amplification Kit is a short tandem-repeat multiplex PCR assay that amplifies 9 unique STR loci and the amelogenin gender-determining marker in a single PCR amplification. This kit is intended for use in human cell identification in various research-based applications requiring human cell discrimination and identity confirmation, such as authentication testing of human cell culture lines and genetic confirmation testing of induced pluripotent stem cells (iPSC).

The AuthentiFiler™ PCR Amplification Kit is:
• An efficient, low-cost PCR amplification kit designed to confirm the genetic identity/source of a human cell
• Performance tested with gDNA extracted from cell pellets, cell culture enrichment media, and formalin-fixed paraffin embedded (FFPE) samples using various gDNA extraction methods and kits
• Highly sensitive—detect low level cross contamination or genetic aborations in your cell lines
• Highly discriminatory, enabling a high level of genetic discrimination between closely related cells, with a small amplicon size that aids in loci recovery from challenging samples (e.g., samples with degraded gDNA, such as FFPE samples)

For Cell Line Authentication Use excluding, Forensic, Paternity, Diagnostic, and Therapeutic applications.

Specifications

Amplicon Size: <300 bp
For Use With (Equipment): 3130 Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, GeneAmp™ 9700, Veriti® Thermal Cycler
Label or Dye: FAM™, LIZ™, NED™, PET™, VIC®
Matrix and Size Standards: Matrix Standard: DS-33 Matrix Standard Kit (Dye Set G5), Size Standard: GeneScan&trade 600 LIZ® Size Standard v2.0
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Line: Applied Biosystems™, AuthentiFiler™
Product Size: 50 tests
Reaction Format: SuperMix or Master Mix
Sample Volume: 25 µL⁄reaction
Technique: STR (Short Tandem Repeat) Analysis

Contents & storage

Store at -15 to -25 °C on receipt and 2 to 8 °C after initial use.

Documents

Manuals & protocols

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