POROS® GoPure™ HQ Pre-packed Column, 0.5 x 5 cm, 1 mL

Catalog number: 4481315

Applied Biosystems™  Related applications: Chromatography | Protein Purification & Isolation

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Description

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS® ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS® high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ HQ 50 µm Column has a quaternized polyethyleneimine functional group for strong anion exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS® resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS® resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS® anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS® Perfusion Chromatography® resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Individual
Column Type: Anion Exchange
Product Size: 1 column
Particle Size: 50 micron
Immobilized Phase: Quaternized Polyethyleneimine
Sample Type (Specific): Cell Culture Supernatants, Serum, Biopharmaceutical Purification Sample
For Use With (Equipment): FPLC
Maximum Operating Pressure: 30 bar
High Throughput Compatibility: Automated Protocols

Contents & storage

20% ethanol, 2 to 30 °C

Documents

Manuals & protocols