Notice
: The Ion PGM™ 200 Sequencing Kit v2 will be discontinued in Q1 of 2017. The supported replacement kit, the Ion PGM™ Hi-Q™ View Sequencing Kit (A30044), is now available for order. The final build of the Ion PGM™ 200 Sequencing Kit v2 will be Q1 of 2017 and will have a 7–8 month shelf life when stocked. Based on in-stock availability, this will allow for migration to the Ion PGM™ Hi-Q™ View Sequencing Kit (A30044) until the end of 2017.
 

Ion PGM™ Sequencing 200 Kit v2

Catalog number: 4482006

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Ion PGM™ Sequencing 200 Kit v2 is an integral component of the Personal Genome Machine™ sequencing platform and single-day sequencing workflow, designed to sequence templated Ion Sphere™ particles deposited in Ion 314™, Ion 316™, or Ion 318™ chips. This sequencing kit should be used in conjunction with Ion OneTouch™ 2 template preparation.

The Ion PGM™ Sequencing 200 Kit v2 provides you with:

• Simple and natural sequencing chemistry
• Low-cost standard reagents for lower cost per sample
• Sequencing read lengths of 200 base pairs with short run times (up to 6 hours)
• Support for Ion 314™, 316™, and 318™ chips
• Support for multiplexed samples

The Ion PGM™ Sequencing 200 Kit v2 contains sufficient reagents for twelve 100 bp or eight 200 bp sequencing reactions using the Ion PGM™ Sequencing System. Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents.

Note: The Ion PGM™ Sequencing 200 Kit v2 should be used with Ion PGM OT2 Template 200 Kit. Old kits should not be mixed with new kits.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Percentage: 10 %
Wells: 26 wells
Gel Type: Bis-Tris Gel
Separation Range: 3.5 kDa to 160 kDa (MES Buffer), 15 kDa to 260 kDa (MOPS Buffer)
Gel Size: Midi (8 cm x 13 cm)
Well Format: 1D Well
Thickness: 1.0 mm
System: NuPAGE® Novex®
Adapters: No Adapters
Quantity: 10 gels
Product Size: 10 gels (1 box)
Mode of Separation: Molecular Weight
Pipette Compatibility: Standard multi-channel pipettor
Sample Loading Volume: 15 µL
For Use With (Equipment): XCell4 SureLock™ Midi

Contents & storage

The Ion PGM Sequencing 200 Kit v2 contains reagents for 12, 100 bp or 8, 200 bp sequencing reactions using the Ion PGM™ system.

Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents.

Store components at -20°C, 4°C, or room temperature, as appropriate.

Documents

Manuals & protocols