Ion AmpliSeq™ RNA Library Kit

Catalog number: 4482335

Ion Torrent™  Related applications: Epigenetic Sequencing | Ion Torrent™ Next-Generation Sequencing | RNA Sequencing

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Description

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.thermofisher.com/confirmrna.

Key Features:

• Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels
• Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA
• Ion AmpliSeq™ RNA Library Kit contains 3 modules:
         -- Ion AmpliSeq™ Library Kit v2
         -- Reverse Transcriptase (RT) Module with SuperScript® VILO™ RT
         -- Magnetic Bead Clean-up Module with Dynabeads®
• Strip-tube and 96-well plate based workflow allow easy sample management
• Compatible with Ion Xpress™ Barcode modules
• Available as 8, 96 & 384 (4x96) reaction kits

Scalable Multiplex PCR Reactions
The Ion AmpliSeq™ RNA technology enables the surveying of gene expression profiles from over 20,000 targeted genes with thorough coverage of the coding genome, starting with as little 500 pg of unfixed RNA or 5 ng of FFPE RNA. The kit is compatible with the Ion AmpliSeq™ RNA ready-to-use or customer-designed panels when ordered thru AmpliSeq.com. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, minimizing unwanted side effects of residual primers in the reaction.

Barcoded Library Preparation
The Ion AmpliSeq™ RNA Library Kit includes reagents for generating amplicons with Ion AmpliSeq™ primers and preparing libraries from the resulting amplicons. The libraries are ready for downstream template preparation using clonal amplification on Ion Sphere™ particles.

The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapter kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost, allowing for accurate sample-to-sample comparisons.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion PGM™ System
Library Type: Targeted Sequencing Library
Workflow Step: Library Generation
Starting Material: Total RNA
Sequencing Type: Transcriptome Sequencing
Species: Human
Technique: Amplicon Sequencing
Product Size: 8 reactions
Multiplex Capability: Compatible with Ion Xpress™ Barcode Kits
Sample Type (Specific): Total RNA, Tissue (FFPE)
Starting Material (Amount): 500 pg of total RNA from fresh frozen tissue or 5 ng of RNA from FFPE samples

Contents & storage

Ion AmpliSeq Library Kit 2.0
1 tube 5X Ion AmpliSeq HiFi Master Mix - Storage: -30 to -10 °C
1 tube FuPa Reagent - Storage: -30 to -10 °C
1 tube Switch Solution - Storage: -30 to -10 °C
1 tube DNA Ligase - Storage: -30 to -10 °C
1 tube Ion AmpliSeq Adapters - Storage: -30 to -10 °C
1 tube Platinum PCR SuperMix HiFi - Storage: -30 to -10 °C
1 tube Library Amplification Primer Kit - Storage: -30 to -10 °C
1 tube Low TE - Storage: Room Temperature

Ion AmpliSeq RNA RT Module
1 tube 5X VILO RT Reaction Mix - Storage: -25 to -15 °C
1 tube 10X SuperScript III Enzyme Mix - Storage: -25 to -15 °C

Ion AmpliSeq DynaBeads Cleanup Module
1 tube Binding Solution Concentrate - Storage: Room Temperature
1 tube Wash Solution Concentrate - Storage: Room Temperature
1 tube Dynabeads Magnetic Beads - Storage: 2 to 8 °C
2 tubes Nuclease Free Water - Storage: Room Temperature
1 Processing Plate & Lid - Storage: Room Temperature

Documents

Manuals & protocols

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