Ion 318™ Chip Kit v2 - FAQs

View additional product information for Ion 318™ Chip Kit v2 - FAQs (4484354)

3 product FAQs found

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What Ion PGM chips are available? How much sequencing data can I expect from each chip type?

The Ion PGM System supports the Ion 3–series chips, including the Ion 314 Chip Kit v2 (Cat. No. 4482261), Ion 316 Chip Kit v2 (Cat. Nos. 4483188 and 4483324), and Ion 318 Chip Kit v2 (Cat. Nos. 4484354 and 4484355). The Ion 314 Chip Kit v2 is available in an 8 pack, while the Ion 316 Chip Kit v2 and Ion 318 Chip Kit v2 are available in 4 packs and 8 packs.

For Ion PGM 3–series chip performance specifications, please see the Ion PGM System Specification Sheet located on the Thermo Fisher Scientific website;https://tools.invitrogen.com/content/sfs/brochures/PGM-Specification-Sheet.pdf

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What Ion PGM chips are available? How much sequencing data can I expect from each chip type?

The Ion PGM System supports the Ion 3 series chips, including the Ion Torrent Ion 314 Chip Kit v2 (Cat. No. 4482261), Ion Torrent Ion 316 Chip Kit v2 (Cat. Nos. 4483188 and 4483324), and Ion Torrent Ion 318 Chip Kit v2 (Cat. Nos. 4484354 and 4484355). The Ion 314 Chip Kit v2 is available in an 8-pack; the Ion 316 Chip Kit v2 and Ion 318 Chip Kit v2 are available in 4-packs and 8-packs.

For more details, please see the Ion PGM System Specification Sheet (https://tools.thermofisher.com/content/sfs/brochures/PGM-Specification-Sheet.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.