Yfiler™ Plus PCR Amplification Kit, 100 reactions - FAQs

How can knowledge of the ratio of male to female DNA present in a mixed sample be used to determine the best HID analysis kit for the sample?  

Knowing the ratio of male to female DNA present in a mixed sample can help the analyst to decide whether autosomal or Y-STR analysis will provide the maximum amount of information on the male component. Generally, for samples with a male:female DNA ratio up to 1:10, both male and female components can usually be visualized and interpreted using an autosomal STR system such as the Quantifiler Duo DNA Quantification Kit. For samples with a male:female DNA ratio above 1:10, the minor component may be below the level of detection of the instrument platform if analyzed using an autosomal STR kit, and therefore the male component may best be analyzed using a Y-STR system such as the Yfiler Plus kit. Each laboratory should determine the minimum level of detection for the male component of a mixed sample using an autosomal amplification system and identify the cut-off value at which Y-STR analysis may prove more successful. This can be achieved as part of an internal validation study of the Quantifiler Duo DNA Quantification Kit.