Thermo Scientific™

POROS™ CaptureSelect™ hGH Affinity Column, 4.6 x 100 mm

Catalog number: 4485169
Thermo Scientific™

POROS™ CaptureSelect™ hGH Affinity Column, 4.6 x 100 mm

Catalog number: 4485169
Catalog Number
4485169
Unit Size
1 column
Price (USD)
3,385.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44851691 column
3,385.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS™ CaptureSelect™ hGH HPLC columns incorporate the advantages of high-speed Poros chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three-minute quantitation of human growth factor (hGH).

Broad utility—POROS™ A, G, and CaptureSelect™ affinity ligands address most biological drug classes
Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins, fusion proteins
Hands-off operation—HPLC method can be automated for streamlined operation
Robust—allows for high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ hGH affinity ligand and POROS™ 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid titer determination and small-scale purification of hGH.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS™ affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

Specifications

Quantity
1 Column
Stationary Phase
CaptureSelect™ hGH
Column Type
Affinity, Growth Hormone Affinity Ligand, Human Specific
Form
Pre-packed Column
Product Line
CaptureSelect™, POROS™
Target
Protein
Type
Affinity Column
Capacity (Metric)
1.7 mL
Matrix
POROS
Elution Volume Max
29 column volumes
Elution Volume Min
14 column volumes
For Use With (Equipment)
FPLC
High-throughput Compatibility
Automated Protocols
Max. Operating Pressure
2600 psi, 180 bar
Particle Size
20 micron

Contents & Storage

Column includes EZ grip fittings and column test certificate.
Store at 2–8°C. DO NOT FREEZE.

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